银杏内酯B对中脑神经细胞损伤的保护作用

Protective effect of ginkgolide B on midbrain nerve cells dameged by kainate in vitro

目的探讨银杏内酯B对海人藻酸(KA)诱发中脑神经细胞毒性作用的影响及可能机制。方法原代培养胚胎中脑神经细胞,分为对照组(A组)、神经生长因子(NGF)50μg/L组(B组)、银杏内酯B 50μg/L组(C组)、KA 100μmol/L组(D组)、NGF 50μg/L+KA 100μmol/L组(E组)、银杏内酯B 50μg/L+KA 100μmol/L组(F组)。MTT法检测细胞活性,改良Lowry法测定中脑神经细胞蛋白质含量,Giemsa染色检测中脑神经细胞集落分化率。结果 A组细胞活性、中脑神经细胞蛋白质含量、集落分化率均低于B组和C组(P<0.05),但高于D组(P<0.05)。与D组相比,E组和F组中脑神经细胞蛋白质含量和集落分化率增加(P<0.05)。结论银杏内酯B能有效对抗KA对中脑神经细胞的毒性作用;其作用机制可能与银杏内酯B维持细胞蛋白质相对含量有关。

Objective To investigate the effect and underlying mechanism of ginkgolide B on the neurotoxicity of midbrain nerve cells induced by kainate.Methods Embryonic midbrain nerve cells were cultured in vitro and divided into six groups of A（normal control）,B（treated with NGF 50μg/L）,C（treated with ginkgolide B 50μg/L）,D（treated with kainate 100mol/L）,E（treated with NGF 50μg/L plus kainate 100mol/L）and F（treated with ginkgolide B 50μg/L plus kainate 100mol/L）.The cell viability and protein content of midbrain nerve cells were detected by MTT and modified Lowry method,respectively.The ratio of colony differentiation was determined by Giemsa staining.Results The cell viability,protein content of midbrain nerve cells and ratio of colony differentiation in group A were lower than those in groups of B and C（P〈0.05）,but higher than those in group D（P〈0.05）.Compared with group D,the protein content of midbrain nerve cells and ratio of colony differentiation were significantly increased in groups of E and F（P〈0.05）.Conclusion Ginkgolide B can effectively protect the midbrain nerve cells from kainate neurotoxicity probably via maintaining the relative protein content.