血管内皮生长因子和缺氧诱导因子-1α在妊娠高血压综合征患者胎盘组织中的表达

Expression of VEGF and HIF-1α in Placentas of Pregnancy Induced Hypertension Syndrome Patients

目的:探讨胎盘组织中血管内皮生长因子(VEGF)和缺氧诱导因子-1α(HIF-1α)表达水平与妊娠高血压综合征(PIH综合征)患者严重程度的相关性。方法:采用免疫组织化学EliVision法对35例PIH综合征患者和40例正常产妇(对照组)胎盘组织中VEGF和HIF-1α的表达水平进行检测分析。结果:VEGF和HIF-1α在胎盘滋养细胞和血管内皮细胞中均有的表达,VEGF在轻度PIH综合征患者胎盘中的表达水平与对照组相比无显著差异,VEGF在中度和重度PIH综合征患者胎盘中的表达水平则较对照组显著下降(P<0.05),HIF-1α在PIH综合征组患者胎盘中的表达水平显著高于对照组(P<0.05),且重度高于中度、中度高于轻度。结论:PIH综合征患者胎盘中VEGF的表达水平降低,而HIF-1α的表达水平升高,并均与病情的严重程度相关,提示其可能参与调控PIH综合征的发生和发展过程。

Objective： To explore the correlation between the expression levels of vascular endothelial growth factor （VEGF）/hypoxia inducible factor-1α （HIF-1α） and pathogenesis of pregnancy induced hypertension （PIH）. Methods： The semi-quantitative immunohistochemical EliVision analysis was used to detect the expression of VEGF and HIF-1 a in placental tissues of 35 PIH patients and 40 normal pregnant women. Results： The expression of VEGF and HIF-1α was observed in trophoblast cells and vascular endothelial cells. The VEGF expression level of mild PIH group showed no significant difference; whereas its expression levels of moderate and severe PIH groups were significantly decreased compared with that of control group （P〈0.05）. The HIF-1α expression levels of mild, moderate and severe PIH groups were significantly increased compared with control group （P〈0.05）; futhermore, the HIF-1α expression level of moderate group was remarkably higher than that of mild group （P〈0.05）, but obviously lower than that of severe group （P〈0.05）. Conclusion： The VEGF expression level in placentas of PIH patients was decreased, while the expression level of HIF-1α was increased, indicating that VEGF and HIF-1α might be involved in the pathogenesis of PIH.