hTSLP在子痫前期孕妇胎盘中的表达及意义

Study the Expression of Human Thymic Stromal Lymphopoietin in Maternal Placenta in Patients with Pre-Eclampsia

目的:通过研究人胸腺基质淋巴细胞生成素(hTSLP)在正常及子痫前期孕妇胎盘中表达的差异,探讨hTSLP在母胎界面免疫耐受中可能发挥的调节功能,及其在子痫前期发病中的作用。方法:收集剖宫产分娩的正常、轻度及重度子痫前期产妇胎盘各10例,分别采用实时荧光定量PCR技术、蛋白质印迹法及免疫组化方法分析hTSLP在正常及子痫前期产妇胎盘组织中的表达情况。采用t检验及Fisher's精确检验进行统计学分析。结果:1重度子痫前期组胎盘组织中hTSLP mRNA及蛋白表达均低于对照组及轻度子痫前期组(P<0.05),轻度子痫前期组与对照组差异无统计学意义(P>0.05)。2hTSLP蛋白在直接接触母体血流的合体滋养细胞的胞浆中表达。结论:hTSLP作为一种免疫调节因子,可能参与母胎界面免疫调节,其缺乏时,可能与重度子痫前期的发病有关。

Objective：To research difference on the expression of human thymic stromal lymphopoietin （hTSLP） in placenta between normal pregnant women and pregnant women with pre-eclampsia and to explore the effects of hTSLP in pre-eclampsia pathogenesis and its potential regulatory function on mater- nal-fetal interface. Methods： Women with uncomplicated pregnancies （10 cases）were included as control group, pregnant women with mild preeclampsia（10 cases）and severe pre-eclampsia（ 10 cases）were included as study groups. The mRNA of hTSLP was quantified by qReal-time PCR;the hTSLP proteins were detected by Western-blot assay and immunohistological analysis. Data were stastically analyzed by t test and Fisher＇s exact test. Results：①ln severe pre-eclampsia group, the expression of hTSLP mRNA（ P 〈 0. 01 ）and protein （ P 〈0. 05）in placenta were significantly lower than those in control group and in mild pre-eclampsia group ;and there was no significant difference between control group and mild pre-eclampsia group（ P 〉0. 05）. ②lmmuno- histological analysis showed that the expression of hTSLP in placenta were restricted in the cytoplasm of cells which were located at the matemoplacental interface（syncytiotrophoblast）. Conclusions：As an immunomodu- latory factor,hTSLP may play an important role in immune tolerance on maternal-fetal interface,and its defi- ciency maybe involved with the pathogenesis of severe pre-eclampsia placenta.