基底细胞乳头状瘤MMP-1 PTEN蛋白的表达及与皮肤光老化关系

Expression of MMP-1 and PTEN protein in the lesions of basal cell papilloma and its correlation with skin photoaging

目的:研究基底细胞乳头状瘤(basal cell papilloma,BCP)患者曝光部位皮损、非曝光部位皮损以及正常皮肤组织中MMP-1和PTEN蛋白的表达,探讨BCP的发病与皮肤光老化之间的关系。方法:选取曝光部位皮损50例,非曝光部位皮损50例,正常皮肤30例。采用免疫组织化学Elivison法检测各组MMP-1和PTEN蛋白的表达情况,比较各组间差异。选取曝光部位皮损90例,分为3组,分别为外用遮光剂二氧化钛霜组、安慰剂组和未用药组,用药3个月后比较3组皮损MMP-1的表达。结果:曝光部位皮损MMP-1的表达评分较正常对照组显著升高(P<0.01),非曝光部位皮损MMP-1的表达评分与对照组无显著性差异(P>0.05)。曝光部位皮损和非曝光部位皮损PTEN蛋白的表达评分均较正常对照组显著降低(P<0.01)。3个月后,外用二氧化钛霜组MMP-1的表达评分较未用药组明显降低(P<0.05)。结论:MMP-1在曝光部位的BCP中呈现高表达。PTEN蛋白在曝光部位和非曝光部位的BCP中呈现低表达。紫外线照射导致的皮肤光老化可能是曝光部位BCP发生的一个诱发因素。

Objective：To investigate the expression of MMP-1 and PTEN protein in basal cell papilloma （BCP）, as well as their correlation with skin photoaging. Methods：Immunohistochemistry technique via Elivison method was employed to measure the expres-sion of MMP-1 and PTEN protein in lesions from 50 cases of BCP on exposed areas, 50 cases on non-exposed areas, and 30 normal controls. We compared the differences among the three groups and analyzed the result. A total of 90 BCP cases on exposed areas were randomly divided into three groups. Titanium dioxide cream and placebo were respectively applied in the trial groups twice daily for 12 weeks, whereas the control group was non-administered. After 12 weeks, the MMP-1 in the lesions of the three groups was measured and compared. Results：The expression scores of MMP-1 on exposed areas were significantly higher than those in the control group （P〈0.01）. No significant difference was found between non-exposed areas and the control group （P〉0.05）. The expression scores of PTEN protein on exposed areas and on non-exposed areas were significantly lower than that in control group （P〈0.01）. The expression scores of MMP-1 in the group that used titanium dioxide were evidently lower than those in control group after 12 weeks （P〈0.05）. Conclu-sion：MMP-1 is overexpressed in BCP on exposed areas. PTEN protein is underexpressed in BCP of exposed areas and non-exposed ar-eas. Skin photoaging is a possible cause of BCP on exposed areas.