基于第二代测序技术的基因资源挖掘

Excavation of Genic Resources Based on Next Generation Sequencing Technologies

转录组研究一直是生命科学研究的一个重要方向,在第二代测序技术问世以前,已经产生了一些行之有效的转录组研究方法,但这些方法存在一定的局限性。第二代测序技术的出现不仅使转录组研究很快进入了高速发展期,同时也为遗传资源的挖掘提供了一套全新的技术平台。本文简要介绍了第二代测序技术的化学原理和特性,重点阐述了利用第二代测序技术进行转录组测序,从而在此基础上挖掘遗传资源的研究。

Transcriptome research has been an important part of life science research. Several effective methods used in transcriptome research have been developed before the advent of next-generation sequencing technologies. However, these methods have some limitations in transcriptome researches. The advent of next-generation sequencing technologies not only prompt transcriptome researches into a high-speed development period but also introduce a whole new technology platform into studies of excavating genetic resources. This review briefly introduced the chemical principles and features of next-generation sequencing technologies and focused on summarizing the excavation of genetic resources based on the transcriptome sequencing using next-generation sequencing technologies.