鱼腥草组培体系优化及耐低温突变体的筛选

Optimization of Tissue Culture System and Low Temperature Resistant Mutants Screening of Houttuynia cordata

为解决豫北地区没有鱼腥草栽培,缺乏耐低温能越冬材料的需求,本实验以鱼腥草茎段为外植体进行不同植物生长调节剂及其浓度的组织培养诱导侧芽发生,优化了鱼腥草侧芽组织培养体系;使用不同浓度的甲基磺酸乙酯(EMS)诱变侧芽,诱导耐低温突变体,通过测定诱变材料可溶性糖含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性变化鉴定材料耐寒性。结果表明茎段侧芽诱导的最佳植物生长调节剂组合培养基为MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1;诱变处理的最佳组合为0.4%EMS处理6 h,获得8株耐低温突变体;侧芽生根的最佳浓度组合为1/2MS+NAA 0.8 mg·L-1。

To solve the requirements for the favorable variant ofHouttuynia cordata which could normally wintering and no H. cordata was planted in north Henan. The subterranean stems of H. cordata were employed for the purpose of inducing lateral bud by tissue culture to optimize H. cordata bud tissue culture method and the lateral buds were treated with ethyl methane sulfonate （EMS） at different concentration to induce low temperature resistant mutants, and then resistant mutants were selected with low temperature. Cold resistance was identified by determining mutagenic material soluble sugar content, changes in activities of catalase （CAT） and superoxide dismutase （SOD）. The best hormone combination medium of bud induced was MS＋6-BA 2.0 mg·L-1＋ NAA 0.2 mg·L-1; The best combination of mutagenic treatment was 0.4% EMS for 6 h; Low temperature resistant mutants materials were obtained. The suitable roofing culture medium was 1/2MS＋NAA 0.8 mg·L-1.