摘要
通过对两种miRNA提取方法——一步法和多步法进行比较研究,以期获得较高质量的蒙古黄芪不同器官的miRNA。实验结果表明,两种方法均可用于蒙古黄芪miRNA提取,二者存在着不同的优缺点,多步法提取成功率较高但步骤繁琐,相比之下一步法实验条件要求严苛但步骤简单、快捷省时,提取的蒙古黄芪miRNA完整性好,可以满足荧光定量PCR等进一步实验需要。
In order to obtain better quality miRNA in different parts of Astragalus membranaceus var. mongholicus, two miRNA extraction methods （one-step method and multi-step method） were compared. The results showed that these two methods had different advantages and disadvantages, but both of them could be used for miRNA extraction. Multi-step method had higher success rate with cumbersome steps, one-step method needed strict experimental conditions but fast and time-saving, the miRNA isolated with one-step method had high integrity which could be used for quantitative RT-PCR and so on.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第8期1255-1258,共4页
Plant Physiology Journal
基金
国家自然科学基金(81373963和30925004)
关键词
miRNA提取
蒙古黄芪
一步法
多步法
miRNA extraction
Astragalus membranaceus var. mongholicus
one-step method
multi-step method
