输入性恶性疟原虫Pf60.1基因多态性初步研究

An Analysis on Pf60.1 Gene Polymorphism of Plasmodium falciparum from Imported Cases

采用PCR方法扩增武汉市2010-2013年经镜检和巢式PCR确诊的101份国外输入性恶性疟病例血样的Pf60.1基因,研究Pf60.1基因多态性。PCR结果显示,共92份血样扩增出3类基因片段,其中52份扩增出313 bp片段,占56.5%;34份扩增出340 bp片段,占37.0%;6份扩增出313 bp和340 bp混合型片段,占6.5%。83份自非洲地区输入病例血样中,46份扩增出313 bp片段,占55.4%,31份扩增出340 bp片段,占37.1%,6份扩增出混合型片段,占7.2%;9份自东南亚地区输入病例血样中,6份扩增出313bp片段,3份扩增出340bp片段。提示输入性恶性疟Pf60.1基因以313 bp基因型较多,并且可能存在多克隆感染情况。

One hundred and one imported falciparum malaria cases in Wuhan City were confirmed by microscopy and Nest-PCR, and the blood samples were collected. The Pf60.1 gene was amplified by PCR. Among 101 blood samples, three Pf60.1 fragments [313 bp（56.5%, 52/92）, 340 bp（37.0%, 34/92）, 313 bp＋340 bp（6.5%, 6/92）]were amplified from 92 samples. Among 83 blood samples from patients returning from Africa, 313 bp fragment were found in 46 samples（55.4%, 46/83）, 340 bp fragment were found in 31 samples（37.1%, 31/83）, and 7.2%（6/83）was mixed-fragment（313 bp＋340 bp）. Among 9 samples from southeast Asia, 6 samples were with 313 bp fragment and3 samples with 340 bp fragment. The results indicated that the most common genotype was 313 bp-genotype, and there would be polyclonal P. falciparum infections.