兔棘阿米巴角膜炎模型血清抗体效价分析

Dynamic Changes of Serum Antibody Titer in a Rabbit Model of Acanthamoeba Keratitis

10只健康新西兰白兔随机分为实验组(n=8)和阴性对照组(n=2)。实验组兔左眼为实验眼,右眼为空白对照眼。实验组采用氢化可的松点眼3 d后行角膜基质内注入赫氏棘阿米巴滋养体和包囊悬液(1×106/ml)0.2 ml。对照组兔角膜基质内注射等量生理盐水。于注射后每天记录兔眼角膜病变情况,刮取角膜组织进行病原学检测。于感染后不同时间采血和摘取角膜进行抗体效价检测和病理学检查。经角膜刮片镜检和角膜病理切片HE染色观察证实,实验眼感染棘阿米巴,并出现棘阿米巴角膜炎典型表现和病理改变。血清抗体效价随感染时间的延长而升高,于感染后第28天达峰值(A450值为2.2358),而后逐渐下降,并维持稳定,直到处死仍高于阴性对照组。阴性对照组血清抗体效价无明显变化。

Ten healthy New Zealand white rabbits were randomly divided into two groups named as experiment group（n=8） and normal control group（n=2）. Left eyes were for experiment, right eyes served as control. New Zealand rabbits were each injected by subconjunctival route with hydrocortisone for three days, and then Acanthamoeba keratitis was induced by intrastromal injection of live Acanthamoeba healyi trophozoites and cysts. Eyes in control group were injected with equivalent volume of physiological saline. Corneal lesions of rabbits were recorded every day after injection,etiological diagnosis was carried out by corneal scraping. Blood samples were examined for serum antibody titer by ELISA. Corneas were removed for pathological examination. Corneal scraping and corneal histopathologic examination proved that experiment eyes were infected by Acanthamoeba, and appeared typical manifestations and pathological changes of Acanthamoeba keratitis. Serum antibody titer raised continuously with infection time and reached the highest level（A450value =2.2358） on the 28 th days post-infection, then began to decline and remained higher level than the control until the rabbits were sacrificed. In control group, no significant change in antibody titer had taken place.