注射用丹酚酸A抗四氯化碳致大鼠肝纤维化的作用

Effect of Salvianolic acid A Injection on CCl4-induced Liver Fibrosis in Rats

目的 观察注射用丹酚酸A抗四氯化碳（CCl4）致大鼠肝纤维化的作用.方法 采用皮下注射CCl4诱导大鼠肝纤维化建模;造模4周后,分为模型对照组和丹酚酸A高、低剂量组及丹酚酸胶囊组,另设正常对照组.高、低剂量组分别尾静脉注射5 mg/kg、2.5 mg/kg丹酚酸A;丹酚酸胶囊组经口灌胃丹酚酸浸膏粉50 mg/kg;正常对照组和模型对照组尾静脉注射生理盐水10 ml/kg;各组每日一次,连续给药4周后,测定血清天冬氨酸转氨酶（AST）、谷氨酸转氨酶（ALT）活性,以及肝脏脏器系数、肝组织羟脯氨酸（Hyp）含量和Ⅰ、Ⅲ型胶原蛋白表达的变化.结果 与模型对照组比,丹酚酸A高、低剂量组和丹酚酸胶囊组均可明显改善纤维化大鼠的体征,显著降低肝脏器系数（P＜0.05）;ALT、AST活性、Hyp含量以及Ⅰ、Ⅲ型胶原蛋白的表达量在丹酚酸A高剂量组显著降低（P＜0.05,P＜0.01）.结论 注射用丹酚酸A具有抗肝纤维化的作用,且静脉注射5 mg/kg丹酚酸A作用优于口服50 mg/kg丹酚酸胶囊.

Objective To investigate the effect of salvianolic acid A（SAA） on CC14-induced liver fibrosis in rats. Methods The liver fibrosis rat model was made by subcutaneous injection of CCl4. After 4 weeks, they were divided into model control group, high and low dose SAA groups, salvianolic acid （SA） capsule group, and normal control group. High and low dose groups were intravenously injected with 5 mg/kg, 2.5 mg/kg SAA, respectively. The SA capsule group was orally administered 50 mg/kg salvianolic acid powder. Model and normal control groups were intravenously injected with 10 ml/kg Saline.The serum AST and ALT levels, liver coefficient, hydroxyproline（Hyp） content, and type I, III collagen expression in liver tissue were detected 8 weeks later. Results Compared with control group, the symptom in high and low doses SAA group and SA capsule group rat were significantly improved, liver coefficient ,ALT and AST activities, Hyp content and type I,III collagen expression were significantly reduced in high doses SAA group （P〈0.05, P〈0.01）. Conclusions Salvianolic acid A injection has anti-Liver fibrosis effects, and the effects with 5 mg/kg SAA injection by intravenous was superior to 50 mg/kg salvianolic acid capsule by oral administration.