乌司他丁对大鼠多脏器功能障碍综合征炎性因子的影响及意义

Influence of Ulinastain on Serum Expression of Inflammation Cytokines in Rat with Multiple Organ Dysfunction Syndrome

目的 通过乌司他丁（UTI）干预多脏器功能障碍综合征（MODS）大鼠,检测外周血炎性因子表达,研究UTI对MODS的可能保护作用及机制.方法 将90只Wistar大鼠随机分为对照组、模型组与UTI组,模型组与UTI组大鼠分别通过尾静脉以15 g/kg注射内毒素（LPS）,UTI组建模前24 h和建模后连续3d,每日2次以1＆#215;105 U/kg腹腔注射UTI进行干预和治疗,观察各时间点各组的体征表现并收集血清用ELISA方法检测TNF-α、IL-1α、IL-4和IL-10的表达水平.结果 ①对照组、模型组和UTI组的死亡率分别为：0％、33％和16％.②TNF-α、IL-1α的表达水平UTI组均低于相对应的模型组.③模型组IL-4的表达水平在6h与24 h与对照组相比明显的升高（P＜0.05）,UTI组与对照组相比在0h、12 h（P＜0.05）,24 h（P＜0.01）显著升高;模型组IL-10的表达水平在12h与对照组相比明显升高（P＜0.05）,UTI组在6 h～12 h时IL-10的表达水平与对照组相比明显升高（P＜0.05）.结论 UTI能降低LPS介导的大鼠MODS的死亡率,可抑制TNF-α、IL-1α释放和促进IL-4、IL-10释放,有效改善MODS的发生发展.

Objective To evaluate the protective effects of Ulinastatin （UTI） on multiple organ dysfunction syndrome （MODS） in rat by detecting serum expression of inflammation cytokines. Methods Ninety rats were randomly divided into 3 groups, the control group, model group and UTI group, model group and UTI group were infused with 15 μg / kg lipopolysaccharide（LPS） in vena caudalis, and the UTI group were intraperitoneally injected with 1 x10^5 U/kg UTI for prevention and therapy before and after the model established. Then the physical sign appearance was observed and the serum was collected to determine the expression of TNF-a, IL-1a, IL-4 and IL-10 by ELISA in each group at different time points. Results O The mortalities were 0%, 33%, and 16% in the control, model and UTI group respectively. ） The expression concentration of TNF-a, IL-1a in UTI group were lower compared with those of model group. The expression concentration of IL-4 in model group has significant increase compared with that of control group at 0 h, 12 h （P〈0.05） and 24 h （P〈0.01）; The expression concentration of IL-10 in model group has significant increase compared with that of control group atl2 h （P〈0.05）, UTI group has significant ascendant compared with control group at 6-12 h has significant ascendant （P〈0.05）. Conclusion The UTI could decrease the mortality of MODS induced by LPS, and it could reduce the increased amplitude of serum levels of TNF-a, IL-1a, whereas enhance the increased amplitude of the serum IL-4 and IL-10, it could also prevent the happen and development of MODS efficiently.