异甘草酸镁对自身免疫性肝炎单核细胞免疫功能影响的实验研究

Experimental study on effects of magnesium isoglycyrrhizinate on immune function of monocytes of patients with autoimmune hepatitis

目的研究异甘草酸镁对自身免疫性肝炎(AIH)患者单核细胞吞噬能力和抗原递呈功能的影响。方法体外培养经密度梯度离心法获得的AIH患者(AIH组)及健康对照者(对照组)的外周血单个核细胞(PBMCs),添加不同浓度异甘草酸镁处理24 h,流式细胞仪测定HLA-DR+和CD80+细胞百分比。将经CD14单克隆磁珠抗体分选提纯后的PBMCs与FITC标记的大肠杆菌共培养,荧光显微镜观察并计算吞噬率和吞噬指数,流式细胞术检测加以验证。结果与对照组比较,AIH组(未加药)PBMCs的HLA-DR+和CD80+细胞百分比、细胞吞噬率及吞噬指数明显降低,差异均有有统计学意义(P<0.01)。在对照组中,与空白组比较,异甘草酸镁组PBMCs的HLA-DR+和CD80+细胞百分比、吞噬率及吞噬指数升高,但差异无统计学意义(P>0.05);在AIH组,与空白组比较,异甘草酸镁组(1、10 mg/mL)PBMCs的HLA-DR+和CD80+细胞百分比、吞噬率及吞噬指数显著升高,差异有统计学意义(P<0.01)。结论异甘草酸镁能够提高AIH患者减弱的单核细胞抗原递呈功能和吞噬能力,提示异甘草酸镁具有调节AIH患者单核细胞免疫功能的作用。

Objective To investigate the effects of magnesium isoglycyrrhizinate on the phagocytic ability and antigen presentation of monocytes of patients with auimmune hepatitis （AIH）. Methods Peripheral blood mononuclear cells （PBMCs） of AIH patients （ the AIH group） and health controls （ the control group） were isolated by the density gradient centrifugation, cultured in vitro, and treated by magnesium isoglycyrrhizinate of different concentrations for 24 h. The percentage of HLA-DR ＋ cell and CD80 ＋ cell was detected by the flow cytometry. PBMCs separated and purified by the GD14 monodonal antibody beads were cultured with fluorescein isothiocyanate （FITC） labeled Escherichia coil The phagocytosis rate and index were observed by the fluorescence microscopy, calculated, and confirmed by the flow cytometry. Results Compared to the control group, the percentage of HLA-DR ＋ cell and CD80＋ cell, phagocytosis rate, and phagocytosis index of PBMCs of the AIH group （not treated by magnesium isoglycyrrhizinate） were significandy lower and the differences were statistically significant （P 〈 0.01 ）. In control group, the percentage of HLA-DR＋ cell and CD80 ＋ cell, phagocytosis rate, and phagocytosis index of PBMCs of the AIH group （treated by magnesium isoglycyrrhizinate） were higher than those of the blank group, but the differences were not statistically significant （P〉0.05）. In AIH group, the percentage of HLA-DR＋ cell and CD80＋ cell, phagocytosis rate, and phagocytosis index of PBMCs of the AIH group （ treated by magnesium isoglycyrrhizinate of 1 and 10 rag/mL）were significantly higher than those of the blank group and the differences were statistically significant （P 〈 0.01）. Conclusion Magnesium isoglycyrrhizinate can improve the weakened phagocytic ability and antigen presentation of monocytes of patients with AIH, which indicates that magnesium isoglycyrrhizinate can regulate the immune function of monocytes of patients with AIH.