G-CSFR IV型异构体在成年急性髓系白血病的表达及其临床意义

Expression of G-CSFR IV Isoform in Adult Acute Myeloid Leukemia and Its Clinical Significance

本研究旨在探讨G-CSFR IV型异构体在成年急性白血病患者中的表达及其临床意义。以19例正常骨髓造血干细胞为对照,利用定量RT-PCR检测正常对照、99例成年AML和34例ALL患者的G-CSFR IV/G-CSFR I型异构体的相对表达水平,并对其中84例AML(非M3)患者的临床特征及化疗效果进行分析。结果表明,GCSFR IV/G-CSFR I相对表达水平在AML患者较ALL患者及正常造血干细胞组高,而G-CSFR IV/G-CSFR I的相对表达水平在ALL患者组与正常造血干细胞组差别无统计学意义。AML(非M3)患者的临床特征及疗效分析显示,G-CSFR IV/G-CSFR I相对高表达的患者比低表达的患者的临床CR率低。G-CSFR IV/G-CSFR I相对表达水平与AML(非M3)患者的性别、年龄、幼稚细胞比例、FAB分型、染色体和融合基因所作的危险分层之间无相关性。结论:G-CSFR IV异构体异常高表达与急性髓系白血病预后差相关。

This study was aimed to investigate the expression of granulocyte colony-stimulating factor receptor IV （ G- CSFR IV） in adult acute leukemia patients and its clinical significance. The bone marrow hematopoietic stem cells from healthy persons were used as controls. The real-time RT-PCR was used to determine the expression level of G-CSFR I - IV in 99 AML, 34 ALL patients and 19 healthy persons. The results showed that the relative expression level of G-CSFR IV/G-CSFR I in AML patients was obviously elevated, as compared with that in ALL patients and controls, while the relative expression level of G-CSFR IV/G-CSFR I in ALL patients showed no statistical difference from controls. The analysis of clinical features and chemotherapentic efficacy demonstrated that the clinical remission rate in patients with high expression of G-CSFR IV/G-CSFR I was lower than that in patients with low expression. The relative expression level of G-CSFR IV/G-CSFR I was not related with risk stratification from sex, age, blast ratio, FAB typing, chromosome and fusion gene. It is concluded that the abnormal high expression of G-CSFR IV relates with poor prognosis of AML.