金雀异黄素对Raji细胞生长抑制作用及机制的研究

Inhibitory Effect of Genistein on the Proliferation of Raji Cells and Its Related Mechanism

本研究旨在探讨金雀异黄素(Genistein,Gen)对BCL-6阳性的Raji细胞系的生长抑制作用及其机制。用台盼蓝染色法及MTT法分析Gen对BCL-6阳性淋巴瘤细胞系Raji细胞的抗增殖作用,应用PI/AV双染法检测Gen对细胞凋亡的诱导作用,Western blot法检测Gen对BCL-6和NCoR表达的影响,免疫共沉淀(Co-IP)法分析Gen对BCL-6和NCoR蛋白相互作用的影响。结果表明:Gen可时间和剂量赖性地抑制Raji细胞的增殖,同时可诱导其凋亡。研究发现,不同剂量Gen对BCL-6和NCoR表达无明显影响,但可抑制BCL-6和NCoR的结合。结论:Gen对BCL-6阳性B淋巴瘤细胞有明显的抑制作用,这种作用有可能成为联合利妥昔单抗和化疗为基础的辅助治疗。

This study was aimed to investigate the anti-proliferative effect of genistein（Gen） on BCL-6 positive Raji cells and its related mechanism. Trypan blue staining and MTT method were used to analyze the anti-proliferative effect of Gen on Raji cells. Cell apoptosis, protein expression and the interaction of BCL-6 and NCoR were detected by PI/AV dual staining, Western blot and Co-IP method, respectively. The results showed that Gen had time- and dose-dependent inhibitory effect on Raji cell proliferation and induced apoptosis. Different dose of Gen had no significant effect on the expression of BCL-6 and NCoR, but Could inhibit the binding of BCL-6 and NCoR. It is concluded that Gen shows inhibitory effect on BCL-6 positive lymphoma cells, which can be as a adjuvant therapy for combined rituximab with chemotherapy.