毛囊单位移植联合原位再生技术修复创面的初步研究

Preliminary Research on Wound Repair by Combining Transplant of Follicular Unit with In-situ Regeneration Technology

目的探讨毛囊单位移植联合原位再生技术对创面修复的作用。方法将24只SD大鼠(雌雄各半)随机分为实验组A(毛囊单位移植组)、实验组B(毛囊单位移植联合原位再生技术组)、对照组C(自体微粒皮移植组)、对照组D(自体微粒皮移植联合原位再生技术组),每组6只,制成SD大鼠体表创面模型,并在制备好的创面上分别覆盖相应的湿润烧伤膏药纱(B、D组)及凡士林纱布(A、C组),每天换药1次。5 d后,创面肉芽组织生长,此时剪取A、B组大鼠的单侧鼠须垫皮肤,并分割鼠须垫毛囊,切取带有毛囊乳头、毛囊周围组织及部分表皮的毛囊单位复合组织,植入已制备好的创面中;从C、D组大鼠的全层皮肤中切取表皮和部分真皮组织,植入已制备好的创面中;A、C组创面覆盖凡士林纱布,B、D组创面覆盖湿润烧伤膏药纱。分别于术后第1、2、3、4、5周取各组创面标本,镜下观察其组织学改变。结果 B组大鼠的创面愈合情况明显优于A、C、D组,组织病理学观察可见,B组创面术后成纤维细胞及新生毛细血管的数量均多于A、C、D组,且其后期减少较明显;B组创面上皮组织生长速度及创面缩小速度均较A、C、D组快。结论湿润烧伤膏能加速实验性大鼠皮肤溃疡创面的愈合,使炎症细胞不断减少,毛细血管和细胞排列有序,调节细胞的数量和形态,促进成纤维细胞逐渐产生胶原纤维,转变成纤维细胞,从而修复创面。

Objective Explore the effect of the combination of follicular unit transplantation andin-situ skin regen-eration technology on wound repair. Method All the 24 SD rats, （ half female and half male） , wererandomly divided into four groups （ 6 rats each group ） ： experiment group A （ follicular unit transplantation group ） , experimental group B＆amp;nbsp;（ follicular unit transplantation combined with in-situ regeneration technology） , control groupC （ autologous micro skin par-ticle transplant group） and control group D （ autologous micro skin particle transplant combined with in-situ regeneration technology）. Wound model was established in every rat and covered by MEBO gauze （group B and D） or vaseline gauze （group A and C） which was changed once aday. Five days later, granulation tissue grew on the wound. At this time, sheared the skin ofone side beardsof rats in group A and B, and separated follicleswhich were then cut into follicular units consisting of follicle papilla, surrounding tissues and some epidermal skin, and the follicular units were transplanted into the prepared wound models of their own. In group C and D, epidermis with part of dermal skin was cut from the full-thickness skin of each rat and transplanted into its own wound model. Vaseline gauzeswere used to cover the wounds in group A and C, MEBO gauzes were used to cover the wounds in group B and D. Tissue samples on wounds in all groups were taken in week 1, 2, 3, 4, 5 after operation, and histological changes were observedunder a microscope. Results The status of wound healing in group B was obviously better than group A, C and D. In view of histopathological observation, newly gen-eratedfibroblasts and capillaries in group B were morethan that in group A, C and D, and their late term decrease were more apparent. The epithelium growth and wound area decrease in group B was faster that that in group A, C and D. Conclusion MEBO can accelerate the wound healing of experimental skin ulcers in rats, by decreasing inflammatory cells, arranging the capillaries and cells in order, regulating the quantity and morphology of cells, and promoting fibroblasts producing col-lagen fibers and turning intofibrocytes thereby repairing the wounds.