氢气饱和等渗盐水腹腔注射治疗兔创伤性关节炎的实验研究

Experimental research on intraperitoneal injection of saturated hydrogen saline for treating traumatic arthritis in rabbits

目的氢气具有抗氧化、抗炎症作用,但对于其治疗创伤性关节炎(traumatic arthritis,TA)的效果鲜有报道。文中旨在研究氢气饱和等渗盐水腹腔注射对兔TA的治疗效果。方法采用随机数字表法将26只新西兰家兔分为正常对照组(n=6)、模型实验组(n=10)和模型对照组(n=10)。模型实验组腹腔注射氢气饱和等渗盐水8mL/kg,每3天1次,模型对照组腹腔注射等量等渗盐水,正常对照组不做任何处理,治疗持续6周。于治疗前后分别取3组家兔血清及关节液测定一氧化氮(nitric oxide,NO)和透明质酸(hyaluronic acid,HA)的含量,结合家兔的行为学表现对治疗效果进行评价。结果模型实验组治疗后行为学有明显改善(P<0.05)。膝关节肿胀评分治疗前各组差异无统计学意义(P>0.05);治疗后模型实验组优于模型对照组(P<0.05)。治疗前2个模型组血清及关节液中NO含量升高,差异有统计学意义(P<0.05),治疗后模型实验组明显下降,其中血清中NO含量由(79.58±13.46)降至(76.23±12.15),关节液中NO含量由(89.45±14.98)降至(80.36±12.78),差异有统计学意义(P<0.05)。治疗后模型实验组血清中HA含量明显下降[(436.82±60.91)vs(340.21±40.57),P<0.05],关节液中显著上升[(1.72±0.37)vs(2.47±0.62),P<0.05]。结论腹腔注射氢气饱和等渗盐水在治疗兔TA方面具有一定的疗效。

Objective Inflammatory response is an important part in pathological change of traumatic arthritis（TA）.Studies show that hydrogen has antioxidate and anti-inflammatory properties, however, there are few reports on treating TA with it.So this research aimed to investigate curative effects of curing traumatic arthritis in rabbits with intraperitoneal injection of saturated hydrogen saline solution . Methods A total of 26 New Zealand white rabbits were divided into three groups：normal control group （n=6）, model experimental group （n=10）, and model control group （n=10）.Rabbits in model experimental group were intraperitoneally injected with 8 ml /kg saturated hy-drogen saline, once every three day;equivalent saline was injected in rabbits of model control group;while no treatment was done on normal control group.The treatment lasted for 6 weeks.Before and after the treatment, the serum and joint fluid of the rabbits in the three groups were respectively taken to determine the content of nitric oxide （NO） and hyaluronic acid （HA）, which was used to evaluate therapeutic effect in combination with the behavioral performance of the rabbits . Results Significant improvement in behavior was found in model ex-perimental group after the treatment （P〈0.05）.No statistical differences were found in knee swelling scores of the three groupsbefore the treatment（P〉0.05）, but model experimental group was superior to model control group in knee swelling scores after the treatment （P〈0.05）.The contents of NO both in serum （[79.58 ±13.46] vs [76.23 ±12.15]） and joint fluid （[89.45 ±14.98] vs [80.36 ±12.78]） in two model groups increased before the treatment, which was of statistical difference （P〈0.05）, but the NO content decreased dramatically in model experimental group after the treatment （[436.82 ±60.91] vs [340.21 ±40.57],P〈0.05）, and no obvious changes were observed in model control group （P〉0.05）.The content of serum HA in model experimental group declined dramatically （P〈0.05） and the joint fluid increased after the treatment （[1.72 ±0.37] vs [2.47 ±0.62],P〈0.05）, while no significant changes were found in model control group before and after the treatment. Conclusion The intraperitoneal injec-tion of saturated hydrogen saline may be effective in the treatment of trau-matic arthritis.