北京市大鼠、小鼠、豚鼠的病理学调查

Pathologic investigation of rats、mice and cavies in Beijing

目的实验动物的健康状况对科学研究的发展有重要的影响,为了保证实验用鼠的健康质量,自从2009年起本实验室对北京市多家实验动物单位的实验用鼠进行了病理学抽检,从病理学的角度来调查北京市部分实验鼠的主要脏器的情况。方法我们主要对实验鼠的心、肝、脾、肺、肾、大肠和小肠进行取材,通过甲醛固定,石蜡切片HE染色、冰冻切片油红"O"染色、PAS染色等方法对实验用鼠进行病理学分析。结果根据病理学的调查结果,部分实验动物在以肝脏和肾脏为代表的器官中出现了不同程度的病变。结论结合对实验鼠的微生物学检测,分析认为,北京市的啮齿类实验动物中几乎无细菌、病毒和寄生虫等病原微生物的阳性感染个体,对人、动物及周围环境无明显危害;但是少部分实验鼠在临床上出现一定程度的病理学异常。

Objective To insure the health of experiment mice , since 2009 our lab tool laboratory rodents from Beijing area to investigate the health status of laboratory rodents .We mainly use the pathologic method to investigate experiment mice.Methods The specimens we took from the experimental animals were mainly hearts , livers, spleens, lungs, kidneys, small intestines and large intestines .Specimens were immediately fixed by formalin .HE staining sections, PAS staining sections , oil red “O” staining frozen sections were observed under light microscope .Results According to the pathological examination there are different degree of pathological changes among the organs of laboratory rodents , such as hepatic and kidney .Conclusion Combined with the result of microbial detection , the laboratory rodents are not infectious with Pathogenic microorganism , such as bacteria , virus and parasite .And they have no harm to human beings , other animals and the surrounding environment .But small parts of laboratory rodents show certain extents pathological anomaly in clinically .