榄香烯注射液联合经皮瘤内注射无水乙醇治疗兔移植性VX2肝肿瘤的研究

An experimental study for the implanted VX2 liver tumor cells in rabbits with PEIT combined with elemene injection

目的探讨超声引导下经皮瘤内注射无水乙醇(percutaneous alcohol injection therapy,PEIT)联合榄香烯注射液静脉给药治疗兔移植性VX2肝肿瘤的疗效及作用机理。方法建立兔移植性VX2肝肿瘤模型30只,随机分为2组:经皮瘤内注射无水乙醇联合榄香烯注射液静脉滴注治疗组(15只);单纯经皮无水乙醇注射治疗组(15只)。实验动物在接种15天后每隔3天治疗一次,5次后处死。手术前、后抽兔耳缘静脉血行血生化检查,MSCT扫描测量肿瘤大小变化,同时进行组织细胞学与细胞超微结构的观察。结果 PEIT+榄香烯注射液治疗组肿瘤增长率与坏死率与单纯PEIT治疗组比较差异均有统计学意义(P<0.05);TUNEL染色法检测显示PEIT+榄香烯注射液治疗组肿瘤细胞凋亡指数明显高于单纯PEIT治疗组(P<0.05);PEIT+榄香烯注射液治疗组血管内皮生长因子(VEGF)表达水平明显低于单纯PEIT治疗组(P<0.05);前者2例出现肝内转移灶,而后者5例出现转移灶,差异性有显著意义(P<0.05);治疗前后两治疗组血生化及肝肾功检查均差异无显著性意义(P>0.05)。结论 PEIT联合榄香烯注射液治疗兔移植性VX2肝肿瘤可明显诱导肿瘤细胞凋亡、抑制VEGF的表达以及肝内转移。

Objective To explore the principle, effect and method of PEIT combined with elemene injection for tumor ablation. Methods Thirty new zealand white rabbits with implanted liver VX2 tumors were randomly divided into two groups： PEIT ＋ elemene injection group （n= 15）, percutaneous ethanol injection （PEI） group （n= 15）. Fifty days after transplantation, the drugs were injected into tumors of all the rabbits once every 3 days. After 5 times of injection, the rabbits were killed. The tumors were measured 3 dimensionally with CT, while the rates of tumor necrosis, the volume of tumor and the growth rate of the tumor were calculated. Liver and kidney function tests （AST, ALT, ALP and BUN） as well as white blood cell counts were assessed before and after treatment, and CT examination was performed at the same time. The morphological and the ultramicrostruetural changes were also investigated under light microscope or electron microscope. Apoptotic cells were evaluated with TUNEL essay, in the same time, vascular endothelial growth factor （VEGF） and expression were examined by immunohistochemistry. Results Among all 2 groups,tumor growing rate for PElT ＋ elemene injection group was the lowest and the necrotic rate was the highest （ P〈0.05）. Apoptotic index （AI） in PEIT ＋ elemene injection group was significantly higher than that in PEIT group a （ P 〈0.05）. The expression of VEGF protein was statistically lowest of the AiDi injection group （ P〈0.05）. There was no evident change of liver and kidney function before and after treatment in the 2 groups （ P 〉0.05）. Conclusion PEIT ＋ elemene injection can induce apoptosis of liver VX2 tumor cells obviously, and inhibit the expression of VEGF of in the liver VX2 tumors tissue.