CD14对生殖支原体LAMPs激活NF-κB的影响

Effect of CD14 on lipid-associated membrane proteins of Mycoplasma genitaliumactivate NF-kappaB

目的探讨CD14在生殖支原体(Mg)脂质相关膜蛋白(LAMPs)激活NF-κB中作用。方法 THP-1细胞分别加入人血清和CD14抗体孵育,提取对数期Mg的LAMPs刺激,ELISA法检测其NF-κBp65水平;采用共转染技术和sCD14预孵育LAMPs,刺激Hela细胞,双荧光素酶报告基因分析CD14在LAMPs激活NF-κB中的作用。结果人血清上调LAMPs诱导THP-1细胞激活NF-κBp65;CD14中和抗体抑制LAMPs诱导THP-1细胞激活NF-κBp65;mCD14和sCD14上调LAMPs诱导Hela细胞激活NF-κB。结论 CD14上调生殖支原体LAMPs激活NF-κB。

To investigate the effects of CD14 on nuclear transcription factorκB （NF-κB） was activated by lipid-associated membrane proteins （LAMPs） of Mycoplasma genitalium （Mg） ,THP-1 cells were pretreated with serum human or CD14 neu-tralizing antibody ,and then were stimulated by LAMPs .The activation of NF-κBp65 was detected by ELISA .After LAMPs was pretreated with sCD14 stimulated Hela cells with the co-transfection ,the activity of NF-κB luciferase were detected by the dual-luciferase reporter gene to analyze the role of CD14-mediated NF-κB activation by LAMPs .The activation of NF-κBp65 was significantly up-regulated in LAMPs activated THP-1 cells by human serum .It’s suggested that CD14 neutralizing anti-body could inhibit the activation of NF-κBp65 in LAMPs stimulated THP-1 .The activation of NF-κB was significantly up-regu-lated in LAMPs activated Hela cells by mCD14 or sCD14 .CD14 could augment the activation of NF-κB by Mg LAMPs .