细粒棘球蚴(Echinococcusgranulosus)CO1与ND1基因序列分析

Gene sequence analysis on CO1 and ND1 of Echinococcus granulosus

目的为了明确内蒙古锡林郭勒盟西乌旗羊株和锡林浩特市人株细粒棘球蚴之间的基因型及其遗传变异情况。方法利用分子生物学技术对采自两地区细粒棘球蚴的线粒体CO1基因与ND1基因进行了克隆和测序,并运用DNAStar5.0中的MegAlign工具对已测出的序列进行了分析。结果西乌旗羊株细粒棘球蚴与锡林浩特市人株细粒棘球蚴CO1基因片段和ND1基因片段大小分别均为936bp和895bp。西乌旗羊株细粒棘球蚴CO1基因序列与新疆羊株细粒棘球蚴的CO1基因序列同源性为99.3%;锡林浩特市人株细粒棘球蚴CO1基因序列与新疆人株细粒棘球蚴的CO1基因序列同源性为98.6%;且西乌旗羊株和锡林浩特市人株细粒棘球蚴ND1基因与国内外已报道的G1型ND1基因序列完全相同。结论表明内蒙古上述两地区细粒棘球蚴的同源性很高,且基因型均为G1型,这为内蒙古某些地区细粒棘球蚴流行虫株的确定提供了重要依据,同时也对当地细粒棘球蚴病的预防和控制具有重要意义。

The purpose of this study was to clarify the strains of E .granulosus from sheep of Xiwuqi and people of Xil-inhot in Inner Mongolia region and its genotypes .CO1 and ND1 of mitochondria gene were cloned and sequenced ,and then they were analyzed by MegAlign of DNAStar5 .0 .Results showed that the length of CO1 and ND1 gene of E .granulosus ,which were from sheep of Xiwuqi or people of Xilinhot ,were 936 bp and 895 bp ,respectively .The homology of CO1 gene sequences of E .granulosus strains from Xiwuqi and Xinjiang was 99 .3% ,while the homology of the corresponding gene sequences of E .granulosus from man of Xilinhot City and Xinjiang were 98 .6% .ND1 gene of E .granulosus of sheep from Xiwuqi and hu-man from Xilinhot were identical to ND1 gene of G1 type .All these indicated that the homology of E .granulosus from the two regions were high and the genotype were G1 type ,which provided an important basis for the determination of strains ,and it al-so had a great significance to prevent and control the disease .