贵州省首例空肠弯曲菌菌血症病例病原鉴定和亚种分型

Campylobacter jejuni isolated from a patient with bacteremia in Guizhou Province,China

目的对贵州省1例菌血症患儿血液中分离的疑似空肠弯曲菌进行鉴定。方法运用传统细菌学方法和分子生物学方法,对从菌血症患者血液分离的可疑空肠弯曲菌进行鉴定和亚种分型。结果来自菌血症患儿血液的可疑菌株,经传统生化鉴定为空肠弯曲菌空肠亚种,特异性多重PCR方法鉴定为弯曲菌属空肠弯曲菌,NAP-mPCR方法鉴定为空肠弯曲菌空肠亚种。结论分离自贵州省菌血症患儿血液的菌株确认为空肠弯曲菌空肠亚种,NAP-mPCR方法可将空肠弯曲菌鉴定到亚种水平。

To identify the isolated suspicious strain of Campylobacter jejuni from the blood of bacteremia patient in Guizhou Province ,China ,conventional and molecular techniques （specific mPCR and NAP-mPCR） were used to identify suspi-cious bacteria strains .Results showed that Campylobacter jejuni suspicious colonies were cultured in bacteremia patient blood samples .The strain was identified as Campylobacter jejuni ssp . jejuni by conventional tests and was identified as Campy-lobacter jejuni by genus specific mPCR .Then the strain was classified as Campylobacter jejuni ssp . jejuni by subspecies NAP-mPCR .The strain was identified as Campylobacter jejuni ssp .jejuni isolated from the blood of bacteremia patient and Campylobacter jejuni can be identified subspecies by NAP-mPCR .