超声辐射力对靶向微泡黏附内皮祖细胞的调控作用

Regulating effect of ultrasound radiation force on the adherence of targeted microububbles to endothelial progenitor cells

目的研究超声辐射力对体外靶向微泡黏附内皮祖细胞的调控作用,为临床治疗动脉粥样硬化不稳定斑块提供前期实验基础。方法首先进行靶向微泡的制备及其黏附内皮祖细胞的拍片,其次建立微血管流体模型,通过高分辨摄像机观测超声辐照力对内皮祖细胞黏附靶向微泡的推移现象。结果镜下观察靶向微泡黏附内皮祖细胞情况较好;超声辐照对微泡无明显破坏作用,推移速度缓慢稳定;当微泡浓度为7×107/ml时,超声辐射力对微泡推移现象较稳定,延长辐照时间对微泡的推移作用无明显影响。结论靶向微泡黏附内皮祖细胞黏附作用较好,超声辐射力可显著提高体外微血管内携带干细胞微泡的靶向黏附作用。

Objective To study the regelating effect of ultrasound radiation force on the adherence of targeted microbubbles to endothelial progenitor cells in vitro and provide preliminary experimental basis for the clinical treatment of unstable atherosclerotic plaques （vulnerable plaques）. Methods Firstly,we prepared targeted microbubble and photographed their adhesion to the endothelial progenitor cells （epcs）. Secondly,microvascular flow model was established. Under the high resolution camera,the process of targeted microbubble adhered to endothelial progenitor cells was recorded. Results Under the microscopy,targeted microbubble adhered to endothelial progenitor cells. The ultrasound radiation force caused no obvious microbubble destruction and drove the targeted microbubbles to move slowly and stably,when the concentration of microbubbles was 7×107/ml. No obvious effect on the microbubble moving was observed by extension of the irradiation time. Conclusion Targeted microbubble can adhere to endothelial progenitor cells. The targeted microbubbles can adhered to endothelial progenitor cells and the adhesion of targeted microbubble carrying stem cells can be significantly improved by the ultrasound radiation force.