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球形孢子丝菌64株由菌丝相向酵母相的转化实验 被引量：1

Gonversion Study of Mycelial to Yeast in S. globosa of 64 Strains
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摘要 目的观察球形孢子丝菌由菌丝相向酵母相转换形态学的变化,在三种培养基的酵母转化率。方法将64株球形孢子丝菌于脑心浸液琼脂(BHI)35℃观察菌落及显微镜下酵母转化形态,并与0.5%葡萄糖脑心浸液琼脂(GSBHI)、马龄薯(PDA)培养基上酵母相转化率进行比较。结果多次传代能使酵母转化更完全,BHI转化率48.43%,GSBHI转化率65.63%,PDA转化率为20.31%。结论球形孢子丝菌由菌丝相至酵母相转化需经过连续多次传代;在GSBHI培养基中35℃酵母转化率最高。 Objective To observe the morphology transformation from mycelia phase to yeast phase of s. globosa and the conversion rate of it in three kinds of culture medium. Methods Total 64-strains of s. globosa were inoculated in the Brain Heart Infusion Agar（ BHI ） in 35℃ and colony of yeast was observed under-microscoped. The result of conversion rate of the yeast was compared with that inoculated with（0. 5% GSBHI） ／ （PDA）. Results High passage can make the yeast conversion more completely. The conversion rate of BHI was 48.43%, GSBHI was 65.63% and PDA was 20. 31%. Conclusion Multiple successive are required to make s. globosa conversion from mycelial phase to yeast phase; and the conversion rate is highest with GSBHI culture medium in 35℃.
作者 景东云 黄欣昕 王学军 彭琳琳 张勤 王玲 吕雪莲
机构地区 大连市皮肤病医院
出处 《中国皮肤性病学杂志》 CAS 北大核心 2014年第9期909-911,923,共4页 The Chinese Journal of Dermatovenereology
关键词 球型孢子丝菌 双相真菌 酵母相 菌丝相 S. globosa Dimorphic fungus Yesst Mycelial
分类号 R379 [医药卫生—病原生物学]
  • 相关文献

参考文献17

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共引文献23

同被引文献22

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引证文献1

二级引证文献7

中国皮肤性病学杂志
2014年 第9期

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