静电纺聚己内酯/壳聚糖纳米纤维膜诱导骨髓间充质细胞成骨分化的研究

Study of Electrospun Polycaprolactone/chitosan Nano Fiber Membrane Induced Bone Marrow Mesenchymal Cells Differentiating into Osteoblasts

目的:观察静电纺聚己内酯/壳聚糖纳米纤维膜对骨髓间充质细胞(BMSCs)粘附、增殖和成骨分化的影响。方法:通过静电纺丝技术制备聚己内酯/壳聚糖纳米纤维膜,将第4代BMSCs接种于纤维膜,扫描电镜、碱性磷酸酶(ALP)和茜素红染色、MTT检测BMSCs粘附、分化及增殖能力。结果:在静电纺丝技术制备的聚己内酯/壳聚糖纳米纤维膜表面,BMSCs的黏附和增殖增强,呈现明显升高的ALP活性,并形成矿化结节,提示具有向成骨细胞方向分化的倾向。结论:静电纺聚己内酯/壳聚糖纳米纤维膜对BMSCs的细胞相容性较好,适合BMSCs的黏附生长,具有诱导其向成骨细胞分化的潜能,有望成为一种新型牙周组织工程支架材料。

Objective： To observe of electrospun polycaprolactone/chitosan nano fiber membrane on bone marrow mesenchymal stem cells （BMSCs） adhesion, proliferation and osteogenic differentiation. Methods： Electrospun polycaprolactone/chitosan nano fiber membrane was prepared by the electrospining technique. The fourth generation of BMSCs were inoculated in the fiber membrane, scanning electron microscope（SEM）, alkaline phosphatase （ALP） and alizarin red staining. MTT was used to the adhesion, differentiation and proliferation of BMSCs. Results.. In the surface of electrospun polycaprolactone/chitosan nano fiber membrane, the adhesion and proliferation of BMSCs were strengthened. It showed that ALP activity was significantly increased and formed the mineralized nodule, which was suggested that the cells had the tendency of differentiating into osteoblasts. Conclusion.. Electrospun polycaprolactone/chitosan nano fiber membrane has favourable cell compatibility. It promotes the adhesion and growth of BMSCs and has the potential of inducing osteogenic differentiation. It is expected to become a new type of periodontal tissue engineering scaffold materials.