串珠素反义cDNA对人喉癌裸鼠移植瘤的抑制作用

Growth inhibition effection of perlecan anti-sense cDNA on human laryngeal carcinoma xnograft in nude mice

目的:研究串珠素(perlecan)反义cDNA质粒(pAP)对喉癌Hep-2细胞裸鼠移植瘤的影响。方法:应用pAP转染喉癌Hep-2细胞,建立人喉癌裸鼠移植瘤模型,观察裸鼠移植瘤的生长速度。分为3组:未转染的Hep-2细胞组(WT组)、空载体phβApr-neol转染组(neo组)及pAP转染组(pAP组)。用RT-PCR和免疫组织化学方法检测各组裸鼠移植瘤的perlecan mRNA和蛋白质的表达情况。结果:肿瘤生长4周后,WT组和neo组裸鼠移植瘤的平均体积较大,pAP组裸鼠移植瘤的平均体积较小,差异有统计学意义(P<0.01)。perlecan mRNA在WT组和neo组高表达,在pAP组低表达,均差异有统计学意义(均P<0.05)。perlecan蛋白在WT组和neo组高表达,定位于癌细胞的细胞核和细胞质,在pAP组低表达,均差异有统计学意义(均P<0.01)。结论:pAP对喉癌Hep-2细胞裸鼠移植瘤的发生、发展有重要影响。

Objective：To observe growth inhibition effectof perlecan anti-sense cDNA（pAP） on human laryn- geal carcinoma xnografted in nude mice. To vertify its antitumor effect and mechanism in vivo, and it may be useful as a biomarker in carcinoma of larynx cancer. Method.. Created the model of human laryngeal carcinoma xnograft in nude mice. To observe growth of those xnografts in nude mice and draw growth curve of xnografted. The expression of perlecan mRNA and portein in xnografts were examined by RT-PCR and immunohistochemistry. Result：Volume of xnografts in the group transfected by the plasmids of pAP were significant small as compared with other two groups made by the wild type cells and phβApr-neol cells （P〈0.05）. It was showed that the expression of perlecan mRNA and protein were significantly reduced in the tumor of pAP transfected Hep-2 cells as compared with the tumors transfected by the wild type cells and phβApr-neol cells （P〈0.01）. Conclusion.. These data raise the possibility that pAP many play key roles in the growth of those xnografts in nude mice.