微波提取工艺对中田大山楂抗氧化物活性的影响

Effects of Microwave Extraction Process on Antioxidant Activities of Zhongtian Crataegus pinnatifida

为探讨微波法提取中田大山楂提取物抗氧化活性的最适条件,比较了乙醇浓度、微波功率、微波时间、液固比对中田大山楂提取物抗氧化活性的影响,并用正交试验对其提取条件进行优化。结果表明:微波功率对提取物抗氧化活性的影响最大,乙醇浓度的影响最小;其提取最佳工艺为乙醇浓度50%,微波功率250W,液固比50∶1,微波3min。在该条件下,提取物的抗氧化活性最佳,浓度为0.146mg/mL的提取液对·OH的清除率为76.67%,对DPPH·的清除率为78.63%,对O-2·的清除率为83.37%。

In order to explore the optimum conditions of extracting antioxidant active substances from Zhongtian Crataegus pinnatifida by microwave method,the effects of ethanol concentration,microwave power,microwave time and solid-liquid ratio on the antioxidant activity were studied,and an orthogonal test was conducted as well.Results：microwave power had the greatest effects on the antioxidant activity and ethanol concentration had the smallest effects on it;the optimum conditions included ethanol concentration 50%,microwave power 250 W,solid-liquid ratio 50∶1 and microwave time 3 min.the clearance rate of 0.146 mg/mL extraction solution on ＆#183;OH reached 76.67%,78.63% on DPPH＆#183; and 83.37% on O-2 ＆#183;.