微波辐射对大鼠窦房结组织HCN4基因表达的影响

Effect of microwave radiation on the expression of HCN4 in rats' sinoatrial nodal tissue

目的观察微波辐射对受损大鼠窦房结(SAN)组织超极化激活环核苷酸门控阳离子通道4(HCN4)基因表达的影响,探讨微波辐射致窦房结损伤的可能机制。方法将60只Wistar雄性大鼠随机分为假辐射组和50 mW·cm-2组,每组30只,建立微波辐射致窦房结损伤大鼠模型,于辐射后1、7、14、28 d及3、6个月,采用原位杂交法检测窦房结HCN4 mRNA表达变化。结果与假辐射组比较,50 mW·cm-2组大鼠于辐射后1、7、14、28 d,窦房结细胞胞质内HCN4 mRNA表达显著增加;而于辐射后3、6个月显著减少,其差异均有统计学意义(P<0.05或P<0.01)。结论HCN4异常表达是微波辐射致SAN损伤的重要致伤分子之一。

Objective To observe the effect of microwave radiation on the expression of hyperpolarization-activated cyclic nucletide-gated cation channel 4 （HCN4） gene in order to investigate the possible mechanism of sinoatrial node （SAN） injury from microwave radiation. Methods Sixty Wistar male rats were randomly divided into non-radiation group and 50 mW· cm^-2 group,with 30 rats in each group. The model of rats＇ SAN injuries resulted from microwave radiation were established. In situ hybridization（ISH） was employed to detect the expression changes of mRNA of HCN4 in SAN after 1 day ,7,14 and 28 days, 3 and 6 months respectively. Results Compared with non-radiation group,the expression of mRNA of HCN4 in the cytoplasm of SAN cells increased significantly in the 50 mW·cm^ -5 group after the radiation for I day and 28 days while it decreased sigui-ficantly after the radiation for 3 and 6 months （ P 〈 0.05 or P 〈 0.01 ）. Conclusion The abnormal expression of HCN4 is one of the important danlage-causing factors of SAN injury from microwave radiation.