三叶因子2和N-myc下游调节基因1在不同子宫内膜组织中的表达

Expression of trefoil factor 2 and N-myc downstream regulated gene 1 in different endometrial tissues

目的 探讨三叶因子2（TFF2）和N-myc下游调节基因1（NDRG1）在不同子宫内膜组织中的表达,为早期诊断子宫内膜癌提供新的生物学指标。方法 收集珠海市人民医院经手术切除并经病理检查确诊为子宫内膜癌组织157例,另选择同期经手术切除并经病理检查确诊的子宫内膜不典型增生组织30例及其他病因手术切除的正常子宫内膜20例,分别检测其TFF2和NDRG1蛋白的表达情况,并分析TFF2和NDRG1蛋白的表达与子宫内膜癌临床病理因素的关系。结果 与正常子宫内膜和子宫内膜不典型增生组织比较,TFF2在子宫内膜癌组织中的阳性表达率显著降低（P〈0.05）,但NDRG1在子宫内膜癌组织中的阳性表达率显著升高（P〈0.01）。TFF2在Ⅰ、Ⅱ期子宫内膜癌组织中的阳性表达率显著高于Ⅲ~Ⅳ期（P〈0.05）;高、中分化子宫内膜癌组织中TFF2的阳性表达率显著高于低分化组织及其他类型（P〈0.01）。与深肌层浸润的子宫内膜癌比较,浅肌层浸润的子宫内膜癌组织中TFF2的阳性表达率显著升高（P〈0.05）;与有淋巴结转移的子宫内膜癌比较,无淋巴结转移的子宫内膜癌组织中TFF2的阳性表达率显著升高（P〈0.01）。而高、中度分化的子宫内膜癌组织中NDRG1的阳性表达率显著低于低分化及其他类型（P〈0.05）。浅肌层浸润的子宫内膜癌组织中NDRG1的阳性表达率显著低于深肌层浸润（P〈0.01）,与无淋巴结转移的子宫内膜癌比较,有淋巴结转移的子宫内膜癌组织中NDRG1的阳性表达率显著升高（P〈0.01）。结论 在子宫内膜组织中,TFF2表达的缺失及NDRG1蛋白的高表达可能与子宫内膜癌的发生发展有重要关系,有望在子宫内膜癌的早期诊断、判断是否存在侵袭转移等临床评估中发挥作用。

Objective To provide new biological markers for early diagnosis of endometrial carcinoma by studying the expression of trefoil factor 2（TFF2） and N-myc downstream regulated gene 1 （NDRG1） in different endometrial tissues. Methods One hundred and fifty-seven cases of endometrial carcinoma which were diagnosed by pathological examination and given resection from Zhuhai People＇s Hospital were chosen,30 cases of atypical endometrial hyperplasia tissue which were diagnosed by pathological examination and given resection were chosen, 20 cases of normal endometrial which were given resection for other reasons were also chosen as control. The protein expression of TFF2 and NDRG1 were tested, the relationship between protein abnormal expression of TFF＇2, NDRG1 and clinical pathology of endometrial carcinoma were analyzed. Results The positive expression rate of TFF＇2 in endometrial carcinoma was lower than that in normal endometrial and atypical endometrial hyperplasia tissue significantly（P 〈 0.05 ）. The positive expression rate of NDRG1 in endometrial carcinoma was higher than that in normal endometrial and atypical endometrial hyperplasia tissue significantly（ P 〈0. O1 ）. The positive expression rate of TFF2 in Ⅰ and Ⅱ period was higher than that in Ⅲ -Ⅳ period（ P 〈 0.05 ）, the positive expression rate of TFF2 in high and medium differentiation endometrial carcinoma was higher than that in low differentiation endometrial carcinoma（ P 〈 O. O1 ）. The positive expression rate of TFF2 in superficial myometrial invasion endometrial carcinoma was higher than that in deep myometrial invasion（P 〈 0.05 ） ;the positive expression rate of TFF2 in endometrial carcinoma with out lymph node metastasis was higher than that with lymph node metastasis（ P 〈0.05 ）. The positive expression rate of NDRG1 in high and medium dif- ferentiation endometrial carcinoma was lower than that in low differentiation endometrial carcinoma and other types （P 〈 O. 05） ;the positive expression rate of NDRG1 in superficial myometrial invasion endometrial carcinoma was lower than that in deep myometrial invasion（ P 〈 0.01 ） ;the positive expression rate of NDRG1 in endometrial carcinoma with lymph node metastasis was higher than that without lymph node metastasis（ P 〈 0. O1 ）. Conclusion The loss of TFF2 and high protein expression of NDRG1 may have an important relationship with pathological mechanism of endometrial carcinoma,it maybe play an important role in the early diagnose and the judgment of invasion metastasis in endometrial carcinoma,