摘要
甘露糖在己糖激酶的作用下形成6-磷酸甘露糖,进一步在磷酸甘露糖异构酶(Phosphomannose isomerase,PMI)的催化下转化成植物可利用的6-磷酸果糖,从而使转化细胞在甘露糖作为主要碳源的培养基上正常生长,而非转化细胞生长受到抑制。这一筛选体系被认为是一种正向筛选(Positive selection),已被成功地应用于重要的粮食作物和经济作物中。甘露糖筛选的植株可以通过多种方法检测,其中氯酚红检测法和试纸条检测法简单方便,适用于初步筛选。甘露糖筛选体系安全高效,已被应用于玉米商业化产品中。综述了甘露糖(mannose)筛选体系的原理、筛选特点、植株的鉴定、筛选方法的优缺点以及在商业化中的应用,列举了利用甘露糖筛选的玉米单性状转化系用于抗鳞翅目和抗鞘翅目昆虫及抗高温淀粉酶的范例及其在育种叠加中的应用。这种育种叠加使得甘露糖筛选的性状与其他性状重叠而得到更广谱的具有抗除草剂抗虫性状的商业化产品。
Mannose can be converted into mannose 6-phosphate in cells and further converted into fructose 6-phosphate in transgenic cells if phosphomannose isomerase（PMI）gene（manA）is introduced as a selection marker. The transformed cells can grow normally on the media with mannose as main carbon source while the non-transformed cells are inhibited to grow. As a positive selection, Mannose / PMI system has been applied to important food crops and important economic crops. The plants selected by mannose can be analyzed through a variety of methods, including simple CPR test and convenient strip test. Mannose selection system is a safe and efficient platform suitable for commercial applications. In this review, advances in mannose selection system with its mode of action, selection characteristics, methods of transgenic plants analysis, advantages and disadvantages in transformation, as well as its applications in transgenic corn production and breeding stacks were summarized. Successful examples to use Mannose / PMI selection system in corn to generate multiple single trait events for insect resistances and high temperature resistant amylase, which have been breeding-stacked with other corn traits selected by different selection systems to provide broad spectrum of herbicide resistance and insect resistance for higher commercial value were presented.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第9期13-21,共9页
Biotechnology Bulletin
