紫杉醇脂质体制备方法优化比较

Comparison on Optimization of Preparation Methods of Taxol Containing Liposomes

为了优化紫杉醇脂质体制备工艺,探究逆向蒸发法、高剪切法、薄膜分散法、注入法及复乳法等脂质体制备方法,对逆向蒸发法制备工艺及制剂处方进行单因素考察,由试验结果得出最优处方为药脂比为1∶25,磷脂含量为2.5%,胆固醇与磷脂比为1∶9,并对包封率以及粒径等指标进行初步评价,最终按照优化后的工艺制得的紫杉醇脂质体的包封率较高,粒径大小符合要求,质量稳定。

To optimize the docetaxel-containing liposome preparation technology, some liposome preparation methods were researched, such as reverse evaporation technique, high shear, film dispersion method, injection and multiple emulsion method. The single factor experiment was adopted to inspect the reverse evaporation technique and prescription preparation. The ideal combination of preparation and formulation were as follows ：taxol-phospholipids=1∶25, phospholipid content 2.5%, cholesterol-phospholipids=1∶9. The reverse evaporation technique and the optimized preparation can be used to prepare paclitaxel liposome successfully.