白粉菌诱导下感病小麦京411中TCTP基因的表达分析

Expression Analysis of TCTP Gene in Susceptible Wheat Jing411 Induced by Powdery Mildew

TCTP广泛存在于动植物及微生物中,参与重要的生物过程。对小麦中克隆到的TCTP基因进行了白粉菌诱导下的表达模式分析,结果发现,在对白粉菌敏感的小麦京411中,白粉菌侵染后TCTP基因应答迅速,且基因表达水平较高。用病毒介导的基因沉默方法沉默京411中的TCTP基因,发现白粉菌成功侵染小麦叶片的比例下降,乳突等抗性结构特征比例上升,H2O2在细胞内的累积水平升高。试验结果表明,TCTP在小麦与白粉菌互作过程中起重要作用。

TCTP plays an important role in biological process, which widely exist in plants, animals, and microorganisms. The expression patterns analysis indicated that TCTP was rapidly and strongly induced by Bgt inoculation in susceptible wheat Jing411. After TCTP gene was silenced by VIGS method, we found that the percentage of succeed Bgt inoculation declined, the proportion of resistant structure increased, such as papilla, and the accumulating level of H2O2 inhanced rapidly. These results indicated that TCTP gene play an important role in wheat-Bgt interaction.