辣椒杂交种纯度鉴定的SSR核心引物筛选

Screening SSR Core Primers for Pepper Germplasm Hybrid Purity Identification

旨在确定一套适于辣椒杂交种纯度鉴定的核心SSR引物,利用17对SSR引物对100份已知辣椒(Capsicum)杂交种进行DNA指纹分析。根据多态性和杂合率两个指标,确定Hpms1-214、Es395和Hpms1-5为辣椒杂交种纯度鉴定的首选核心引物,利用这3个引物进行筛选,97个品种(占97%)能够找到具有杂合带型的鉴定引物,确定5个引物Es330、Es363、Epms923、Es120和Es64为辣椒杂交种纯度鉴定的备选核心引物。筛选出14个品种的特异性引物,可进一步筛选每个辣椒杂交种的双亲互补型引物。

In order to determine a set of SSR core primers fitting for pepper hybrids＇ purity identification, 100 pepper（Capsicum）hybrids were analyzed by 17 SSR primers. According to their polymorpism and heterogosite rate, three primers Hpms1-214, Es395 and Hpms1-5 were determined as preferred core primers for pepper hybrid purity identification. Using three preferred core primers, 97 hybrids（accounting for 97%）have heterozygosis band type in at least one primer. Five primers Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for pepper hybrid purity identification. 14 varieties had specific primers within the 100 varieties. Primers with complementary band types were further screened for pepper purity identification.