紫外、^(60)Co复合诱变选育烟色烟管菌漆酶高产突变株

Screen High-producing Laccase Strain of Bjerkandera fumosa Through UV and ^(60)Co Composite Mutagenesis

对烟色烟管菌(Bjerkandera fumosa 5.0172)的孢子悬浮液进行紫外诱变筛选得到一株诱变株ZW-7,其产漆酶的活力是原始菌株的1.33倍,继代培养5代后,未见酶活下降。将诱变株ZW-7的孢子悬浮液用60Co-γ射线进行辐射诱变筛选得到一株诱变株Co-11,其产漆酶的活力是ZW-7的1.18倍,为380.5 U/L,较原始菌株的250.6 U/L提高了约58.1%,继代培养5代后,酶活稳定。

Mutagenic strains ZW-7 was obtained from the spore suspension of Bjerkandera fumosa（5.0172）by UV mutagenesis and screening. The laccase activity was 1.33 times than the original strain. After subcultured five generations, laccase activity was stable. ZW-7 was mutagenized to a new mutagenic strain Co-11 by treating with ^60Co-γ ray radiation and its activity was 380.5 U/L, which was 1.18 times than ZW-7 and 1.58 times than the original strain（250.6 U/L）. After subcultured five generations, enzyme activities of Co-11 remains stable.