斑玉蕈不同交配型的原生质体单核菌株差异分析

Variance Analysis of Different Mating Type Strains in Mononuclear Protoplast of Hypsizygus marmoreus

对2个斑玉蕈菌株进行了原生质体分离与交配型鉴定,并对不同交配型单核体的代谢特性进行了研究。结果表明,2个斑玉蕈菌株均出现2种交配型,2种交配型比例不一致,且2个菌株的两种单核体的分离比例均不符合1∶1分离比,交配型比例上出现明显的偏差现象。2个菌株中,不同交配型单核菌株的菌丝形态有明显差别,代谢过程中的菌丝生物量及发酵液的pH、还原糖含量和电导率均存在差异,表明在这2个菌株中不同交配型单核菌株的菌丝形态及代谢特性与交配型因子相关。

Tow Hypsizygus marmoreus strains were studied by protoplast isolation and mating-type identification. Metabolic characteristics of different mating type strains were studied. Results showed that each of the 2 Hypsizygus marmoreus strains had 2 mating type. The ratio of two mating-type monokaryons of 2 strains was not 1∶1 and the proportion of mating types had different phenomenon. Different kinds of mating type had different hyphae form and there was difference between the conductivity, the pH, the content of reducing sugar of the broth and the mycelial biomass in different mating type strains, which indicated that hyphae form and metabolic characteristic had relations with mating factors in this two strains.