光纤化学传感荧光法测定维生素B_2片剂含量的研究

Determination of Content of VB_2 Tablet with Fluorescence Fiber-Optic Chemical Sensor

[目的]利用光纤化学传感荧光法,准确测定维生素B2片剂的含量.[方法]利用光纤化学传感荧光法检测系统,将维生素B2片溶解于水溶液中,测定其荧光光谱,定量分析其含量.[结果]该方法维生素B2最大发射波长为533 nm,质量浓度在3.2×10-5～8×10-4 mg/ml与荧光强度呈良好线性关系,检测线为1.55×10-8 mg/ml,日内精密度为0.19％,日间精密度为1.2％,回收率为97.9％ ～105.1％.[结论]采取该方法测定维生素B2药片含量的准确度高,检测线低,结果令人满意.

[Objective] To establish a new method of accurate determination of content of VB2 tablet with fiber optic chemical sensor fluorescence.[Method] Set up optic chemical fiber sensing fluorescence detection system,dissolve VB2 tablets in an aqueous solution,measure spectrum of the solution and get the content of the VB2 tablets.[Result] Using this method,the maximum emission wavelength is 533 nm,VB2 concentration in 3.2 × 10-5-8 × 10-4 mg/ml have a good linear relationship with its the fluorescence intensity.The test line is 1.55 × 10-8 mg/ml.Within-day precision precision is 0.19％.Day to day precision is 1.2％ and the recovery rate is 97.9％-105.1％.[Conclusion] This study shows that the method of determination of VB2 tablets has high accuracy,low test line,robust and satisfactory results.