红松种鳞多酚抗肿瘤作用的研究

Study on the Antitumor Properties of Korean Pine Scale Polyphenols

[目的]研究红松种鳞多酚对癌细胞体外增殖的抑制作用。[方法]分别采用不同浓度红松多酚提取液对人骨髓神经母细胞瘤细胞株SH-SY5Y、人肺腺癌细胞株A549、人皮肤癌细胞株A375、人肝癌细胞株HepG2、人卵巢癌细胞株SKOV3这5种常见的肿瘤细胞进行试验,采用MTT法检测体外细胞增殖抑制率。[结果]红松多酚提取物对SH-SY5Y、HepG2、SKOV3的抑制作用不明显,而对A549、A375均有抑制效果。[结论]在红松多酚提取液固形物含量为0.4 mg/ml时,其对A549细胞抑制效果最佳,在该浓度下,红松多酚提取液对人肺腺癌细胞A549的抑制率可达到55%。

[Objective] To study inhibition effect of Korean pine scale polyphenols on cancer cell in vitro proliferation.[Method] Using different concentration of Korean pine scale polyphenols extract,experiments were conducted on human bone marrow neuroblastoma cell line SH SY5Y and A549 lung adenocarcinoma cancer cells and human skin cancer cell lines A375,human liver cancer cell line HepG2,human ovarian cancer cell lines SKOV3.MTT was used to detect the inhibition rate on in vitro cell proliferation.[Result] Korean pine polyphenols extracts have no obvious inhibition effect on SH-SY6Y,HepG2,SKOV3,while have inhibition effect on A549 and A375.[Conclusion] When the solid content in Korean pine polyphenols extracts is 0.4 mg/ml,the inhibition effect on A549 can up to 55％.