复方唇香草颗粒干预As斑块炎症反应信号通路的实验研究

Experimental study on compound ziziphora clinopodioides lam granule intervening as plaque inflammation signaling pathways

目的：探讨以维吾尔药为主的复方唇香草颗粒对 Apo-E基因敲除小鼠动脉粥样硬化（As）斑块 IκK-β/IκB-α/NF-κB 信号通路的影响及其稳定 As斑块作用的可能机制。方法选择70只6～8周龄 Apo-E基因敲除小鼠，予高脂饮食喂养12 w后，随机处死10只，取主动脉根部，HE染色普通光镜下观察并确定As形成，其余小鼠随机分为模型组（生理盐水）、中药对照组（血脂康）、西药对照组（辛伐他汀）及复方唇香草颗粒高、中、低剂量组，每组10只，给药12 w后处死，采用原位杂交法检测小鼠斑块内 IκK-β、IκB-α和 NF-κB mRNA表达。结果与模型组比较，中药对照组、西药对照组及复方唇香草颗粒高、中、低剂量组 IκK-β和 NF-κB mRNA的表达降低（P ＜0．01），IκB-αmRNA的表达增高（P ＜0．05）；而西药对照组与复方唇香草颗粒高、中剂量组比较，差异均无统计学意义（P ＞0．05）。结论复方唇香草颗粒具有抗 As的作用，其机制可能与调控 IκK-β/IκB-α/NF-κB 信号通路的活动而抑制炎症反应有关。

Objectives To discuss the influence of compound Ziziphora clinopodioides Lam granule aortic atherosclerotic plaques IκK-β/IκB-α/NFκB signaling pathway of Apo-E Knockout mice and the possible mechanism in stabilizing As plaque.Methods 70 6-8 week-old Apo-E knockout mice were given a high-fat diet for 12 weeks,then 10 were randomly killed and their aortic root were taken,the formation of As was observed with HE staining ordinary light microscope.The remaining mice were randomly divided into 6 groups,namely,model group （saline group）,traditional Chinese medicine control group （xuezhikang group）,western medicine control group （simvastatin group）,and the experimental groups （compound Ziziphora clinopodioides Lam granule high,medium and low dose groups）.10 mice in each group were kill-ed after 1 2 weeks′dosing.The expression of IκK-β,IκB-αand NF-κB mRNA in mice plaque was detected with situ hybridization method.Results Compared with model group （saline group）,traditional Chinese medicine control group （xuezhikang group）,western medicine control group （group simvastatin）,the ex-perimental groups （compound Ziziphora clinopodioides Lam granule high,medium and low dose groups） could reduce the IκK-βand NF-κB mRNA expression （P 〈0.01）and increase the IκB-αmRNA expression （P 〈0.05）.The western medicine control group （simvastatin group）and the experimental groups （com-pound Ziziphora clinopodioides Lam granule high,medium dose groups）showed no significant differences （P 〉0.05）.Conclusion Compound Ziziphora clinopodioides Lam granule has the function of against scle-rosis of arterial congee appearance,its mechanism may be related to regulate the IκK-β/IκB-α/NF-κB sig-naling pathway activity and inhibit the inflammatory response.