正畸治疗的年轻恒牙和成熟恒牙的牙髓组织中VEGF水平比较

Expression of VEGF in dental pulp of immature and mature permanent teeth of human

目的探讨正畸治疗的年轻恒牙和成熟恒牙的牙髓组织中血管内皮生长因子(VEGF)水平及其临床意义。方法收集2010年6月—2013年10月在新疆医科大学第二附院口腔科行正畸治疗拔除的无龋健康的第一前磨牙56颗,包括年轻恒牙30颗和成熟恒牙26颗,采用常规免疫组化链酶亲和素-生物素-过氧化物酶复合物(srteptvadin-bitin complex,SABC)法比较年轻恒牙和成熟恒牙牙髓标本中血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达。结果年轻恒牙牙髓成纤维细胞中的VEGF呈强阳性表达,向冠部和根部表达逐渐减弱;成熟恒牙牙髓成纤维细胞中的VEGF呈阳性表达,向冠部和根部表达逐渐减弱。与年轻恒牙比,成熟恒牙牙髓成纤维细胞中的VEGF表达较弱,在冠部和根部的表达较弱。结论 VEGF在人年轻恒牙和成熟恒牙牙髓组织中表达有差异,VEGF参与恒牙牙根的发育与成熟。

Objective To investigate the expressions and distributions of vascular endothelial growth factor （VEGF）in dental pulps of immature and mature permanent teeth of human.Methods The normal teeth, extracted for orthodontic from the Second Affiliated Hospital of Xinjiang Medical Uni versity,including 30 cases of immature permanent teeth and 26 cases of mature permanent teeth,from 2010.6-2013.10,were examined by Srteptvadin-bitin Complex （SABC）to determine the expressions of VEGF in their dental pulps.Results The expression of VEGF was strongly positive in the pulp fibroblasts of immature perma-nent teeth （P 〈0.05）,but gradually weakened in the crown and root canal.The expression of VEGF was positive in the pulp fibroblasts of mature permanent teeth （P 〈0.05 ）,but gradually weakened in the crown and root canal.Compared with mature permanent teeth,the expression of VEGF was stronger in the pulp fibroblasts of immature permanent teeth,weakened in the crown and root canal （P 〈0.05）.Con-clusion The expressions of VEGF in dental pulp of immature and mature permanent teeth showed signifi-cant differences and VEGF was concerned with the growth and maturation of permanent tooth.