芒针透刺对急性脊髓损伤兔脊髓细胞凋亡受体Fas及Caspase-3的影响

Effects of Elongated Needle Therapy on the Expressions of Fas and Caspase-3 in Rabbits With Acute Spinal Cord Injury

目的探讨芒针透刺治疗急性脊髓损伤的可能作用机制。方法 135只日本大耳兔随机均分为假手术组、模型组和芒针透刺组,每组45只。模型组和芒针透刺组建立兔T13-L1急性脊髓损伤模型;假手术组、模型组不给任何刺激,芒针透刺组芒针透刺双侧秩边、水道穴,辅以针刺气海、中极,得气后同侧秩边、水道连接于针灸治疗仪上,刺激时间15min,频率20~40次/min,强度1.5~3.0V,每日1次,共5天。分别于术后1、3、5天取伤段脊髓观察形态学变化,检测脊髓细胞凋亡受体Fas、Caspase-3蛋白及其mRNA表达。结果假手术组各取材点脊髓界限清晰,神经元细胞结构完整;模型组脊髓组织正常结构紊乱,局部区域内神经元细胞可见细胞核消失;芒针透刺组脊髓组织结构及神经元细胞破坏较模型组轻微。与假手术组比较,模型组大鼠术后1、3、5天脊髓Fas、Caspase-3蛋白及mRNA表达明显升高（P〈0.05或P〈0.01）;芒针透刺组术后第3、5天Caspase-3蛋白及mRNA表达明显降低,术后第3天Fas蛋白及mRNA亦降低（P〈0.05或P〈0.01）。结论芒针透刺对急性脊髓损伤有明显的保护作用,抑制脊髓Fas、Caspase-3水平可能是其作用机制之一。

Objective To study the possible mechanism of elongated needle therapy for acute spinal cord injury.Methods Totally 135 Japanese rabbits were randomized into the sham operation group,model group and elongated needle therapy group,with 45 in each.The T13-L1 acute spinal cord injury models were established in the model group and elongated needle therapy group.The sham operation group and model group were not given any stimulation.The elongated needle therapy group was given elongated needling on bilateral points Zhibian（ BL54） and Shuidao（ ST28）,and common acupuncture needling on Qihai（ CV6） and Zhongji（ CV3）.The morphological changes of spinal cord were observed.The expressions of Fas,Caspase-3,Fas mRNA and Caspase-3 mRNA were detected.Results The spinal boundary was clear and neuronal cell structure was intact in the sham operation group.The spinal structure was disordered and the nucleus of neuronal cells was disappeared in the model group.The damage of spinal structure and neuronal cells was slight in the elongated needle therapy group.Comparing with the sham operation group,the expressions of Fas,Caspase-3,Fas mRNA and Caspase-3 mRNA were significantly increased in the model group on the 1st,3rd,and 5th days after operation（ P〈0.05 or P〈0.01）.The expressions of Caspase-3 and Caspase-3 mRNA were significantly decreased in the elongated needle therapy group on the 3rd,and 5th days after operation,and the expressions of Fas and Fas mRNA were significantly decreased in the elongated needle therapy group on the 3rd day after operation（ P〈0.05 or P〈0.01）.Conclusion The elongated needle therapy has an obvious protective effect on acute spinal cord injury,which may be relevant to inhibiting the expressions of Fas and Caspase-3.