摘要
目的建立RP-HPLC同时定量测定丁公藤中东莨菪苷、东莨菪内酯及绿原酸的分析方法。方法丁公藤甲醇超声提取物的分析在Hypersil ODS色谱柱（4.6 mm×200 mm,5μm）实施,乙腈（A）-0.1%磷酸水溶液（B）为流动相系统进行梯度洗脱;柱温30℃;检测波长347 nm;体积流量1.0 mL/min。结果东莨菪苷、东莨菪内酯、绿原酸分别在0.399~31.920μg/mL（r=0.999 9）、0.360~28.800μg/mL（r=0.999 9）、0.412~32.960μg/mL（r=0.999 9）范围内与峰面积呈良好的线性关系;平均回收率（n=6）分别为99.9%（RSD为1.3%）、99.5%（RSD为0.6%）、99.9%（RSD为1.0%）。结论本方法中提取溶剂和检测波长适合同时测定丁公藤中3个成分。
AIM To develop a RP-HPLC method for simultaneously determining scopolin, scopoletin and chlorogenic acid from the stem of Erycibes obtusifolia Benth. METHODS An HPLC analysis of methanol ultrason- ic extract of E. obtusifolia Benth was performed on a Hypersil ODS （4.6 mm ×200 mm, 5 μm） with the mobile phase consisting of acetonitrile （A） -0. 1% aqueous phosphoric acid solution （B） in gradient elution mode. The detection wavelength was set at 347 nm and the column temperature was kept at 30 ℃ The flow rate was 1.0 mL/min. RESULTS There was a good linear relationship between the concentration and peak area in the range of 0. 399 - 31. 920 μg/mL, 0. 360 - 28. 800 μg/mL, and 0. 412 - 32. 960 μg/mL for scopolin, scopoletin and chlorogenic acid （ r = 0. 999 9）, respectively. Their average recoveries were 99. 9% （ RSD = 1.3% ）, 99.5% （ RSD = 0. 6% ）, 99.9% （ RSD = 1.0% ）, respectively. CONCLUSION The extraction conditions and wave- length detection of the established method are suitable for the simultaneous determination of three constituents from the stem of E. obtusifolia Benth.
出处
《中成药》
CAS
CSCD
北大核心
2014年第9期1906-1909,共4页
Chinese Traditional Patent Medicine
基金
辽宁省教育厅科研项目(L2010332)
