胰岛素样生长因子-1启动子区域CA重复序列多态性与早产儿视网膜病相关性研究

Study on the correlation between CA repeat polymorphism in the promoter region of insulin-like growth factor-1 and retinopathy of prematurity

目的探讨胰岛素样生长因子-1（IGF-1）启动子区域CA重复序列多态性与早产儿视网膜病（ROP）的相关性。方法将2010年1月至2012年6月在广东省妇幼保健院新生儿科住院期间发生ROP病变的早产儿为病例组,按1∶1的比例选择同期住院追踪至眼底周边视网膜血管化无ROP病变的早产儿为对照组,抽取血液样本进行基因多态性分析。所有研究对象父母均为广东户籍。采用比值比（OR）和95%可信区间（CI）进行风险估计,采用Pearson相关进行直线相关分析。结果病例组和对照组各纳入56例早产儿,性别差异无统计学意义（P〉0.05）。在研究对象中共检测到7种重复序列,（CA）19重复序列最多,占41.1%。携带各种基因型与ROP的风险评估中,携带IGF-1（CA）17、19、21重复序列的OR（95%CI）分别是1.828（0.517-6.457）、1.210（0.678-2.160）、1.251（0.662-2.364）,P均〉0.05。携带各种基因型与ROP的直线相关分析rs值0.012,P〉0.05。结论本研究未发现研究人群的IGF-1（CA）重复序列多态性与ROP具有相关性。

Objective To study the effects of CA repeat polymorphism in the promoter region of insulin-like growth factor-1 （ IGF-1 ） on retinopathy of prematurity （ROP）. Methods Preterm neonates who developed ROP during their hospital stay in the neonatal department from January 2010 to June 2012 of were recruited as ROP group, and preterm neonates without ROP at the same time as the control group. All the parents of the patients were born in Guangdong Province. Venous blood samples were taken, DNA extracted and analyzed. Odds ratio （OR） and 95% confidence interval （95% CI） were used to assess the risk and Pearson correlation for linear correlation analysis. Results 56 patients assigned to each group, and no statistically significant differences on gender（P 〉 0. 05 ）. In ROP group, 7 types of CA repeat were found, and the most frequent one was （CA） 19 repeat with incidence of 41.1% . Preterms carried IGF-1 （CA） 17, 19, 21 repeat had the OR （95%CI） of 1. 828（0.517 - 6. 457 ）, 1. 210（0. 678 - 2. 160） and 1. 251 （0. 662 - 2. 364 ） to develop ROP respectively. Using linear correlation analysis, rs was 0. 012, and no statistically significant differences （ P 〉 0. 05 ）. ConclusionsNo correlation were found between IGF-1 （CA）repeat and ROP in this study.