对氧磷酶1 Leu55Met基因多态性与牙周炎伴糖尿病的相关性研究

Correlation between paraoxonase1 Leu55Met gene polymorphism and chronic periodontitis with diabetes mellitus

目的:研究对氧磷酶(paraoxonase,PON)1 Leu55Met的基因多态性与单纯牙周炎及牙周炎伴糖尿病(diabetes mellitus,DM)(本文特指2型糖尿病)的易感性是否相关。方法:收集慢性牙周炎(chronic periodontitis,CP)伴DM患者、单纯CP患者、单纯DM患者及正常人各50例口腔颊黏膜拭子,采用口腔/咽拭子基因组DNA提取试剂盒提取DNA,然后应用聚合酶链式反应-限制性片段长度多态性检测PON1 Leu55Met的基因型分布。结果:DM伴CP组(CP+DM)、单纯CP组(CP)、单纯DM患组(DM)、正常组(H)在PON1 Leu55Met基因型分布有统计学差异(χ2=12.444,P=0.006)。结论:CP与DM的易感性可能与PON1 Leu55Met基因多态性有关。

Objective ：To study the relationship between paraoxonase 1 （PON1） Leu55Met gene polymorphisms and the risk of chronic periodontitis（CP） with diabetes. Methods：Buccal swab of 50 patients with CP and diabetes,50 patients with CP,50 patients with diabetes and 50 healthy controls were collected. DNA was isolated from buccal swabs of patients with CP and diabetes,CP,diabetes and health controls and were extracted by Chelex-100. PON1 Leu55Met gene polymorphisms were assessed by PCR amplification and digestion with restriction enzyme respectively. Results：There were statistically significant differences in the distribution of PON1 Leu55Met genotype among CP with diabetes group, CP group, diabetes group and health control group（χ^2=12.dd4,P=-0.006）. Conclusion：Susceptibility of CP and diabetes may associate with PON1 Leu55Met polymorphisms.