摘要
以黄瓜品种‘津优4号’为材料,利用人工气候箱进行低温弱光处理(昼/夜,18℃/10℃),研究外源褪黑素(MT)对低温胁迫下黄瓜幼苗生长和抗氧化系统等生理指标的影响。结果显示:低温弱光下,与对照相比,外源褪黑素处理显著提高了黄瓜幼苗株高、茎粗、植株鲜重和干重,叶绿素和根系活力分别显著提高了13.3%和18.8%,抗氧化物质GSH及ASA的含量分别显著增加了39.3%和24.7%,保护酶SOD、POD、CAT、APX活性均显著升高;同时,外源褪黑素处理还显著提高了黄瓜叶片质膜及液泡膜H+-ATP酶的活性,从而使黄瓜幼苗叶片MDA含量和电解质渗漏率分别显著降低了28.7%和29.7%。研究表明,低温弱光下外源褪黑素可通过提高黄瓜幼苗保护酶活性、抗氧化物质的含量、细胞膜ATP酶活性等来降低质膜过氧化水平,保持细胞膜的完整性和功能,从而增强黄瓜幼苗对低温弱光的适应性,维持其正常生长,并以200μmol·L-1褪黑素处理效果较好。
Cucumber cultivar‘Jinyou 4'was subjected to low temperature treatment of 18℃/10℃(day/night)in the artificial climate chamber.The effects of exogenous melatonin(MT)on the growth and antioxidant system of leaves in cucumber under low temperature and weak light stress were investigated.The results showed that:compared with the control treatment,exogenous MT treatment could significantly promote the increment of plant height,wall thickness,flesh weight,dry weight;the chlorophyll content and root activity increased by 13.3% and 18.8%,GSH content and ASA content by 39.3%and 24.7%,respectively and increased SOD,POD,CAT,APX,membrane H+-ATP activities,while reduced MDA content and electrolyte leakage by 28.7% and 29.7%in cucumber seedlings leaves under low temperature and weak light stress.So we can conclude that exogenous MT treatment could raise the activities of antioxidant enzymes and matter,membrane H+-ATP activities,decrease the membrane lipid peroxidation,maintain membrane integrity and function,and improve the adaptability of cucumber seedlings under low temperature and weak light stress,the 200μmol·L-1 MT treatment was better.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第8期1608-1613,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
安徽省自然科学基金项目(1208085QC55)
安徽科技学院引进人才基金项目(ZRC2009250)
关键词
低温弱光
褪黑素
黄瓜幼苗
抗氧化系统
ATP酶活性
low temperature and weak light
melatonin
cucumber seedlings
antioxidant systems
H+-ATP activities