组织蛋白酶-B、组织蛋白酶-L及尿激酶类纤溶酶原激活剂在非霍奇金淋巴瘤中的表达及其意义

Expression of cathepsin B, cathepsin L and uPA mRNA levels in non-Hodgkin lymphoma and their significance

目的 探讨组织蛋白酶-B（CTS-B）、组织蛋白酶-L（CTS-L）及尿激酶类纤溶酶原激活剂（uPA）在非霍奇金淋巴瘤（NHL）中的表达,分析三者间及与临床病理因素间的相关性.方法 选取未经化疗或放疗的NHL患者39例,以淋巴结反应性增生15例作为对照.采用实时荧光定量反转录聚合酶链反应（PCR）法检测淋巴结肿瘤组织CTS-B、CTS-L及uPA mRNA的相对表达水平.分析三者的表达变化及与临床病理因素间的相关性.结果 CTS-B、CTS-L、uPA mRNA在NHL患者中表达水平明显高于对照组（0.403比0.128、0.209比0.057、0.459比0.031,均P＜0.05）,且三者间存在相关性.CTS-B、CTS-L、uPA mRNA在晚期NHL组中表达水平高于早期组（0.453比0.341、0.204比0.085、0.372比0.196,均P＜0.05）,CTS-B、CTS-L、uPA mRNA在NHL化疗敏感组中的相对表达水平低于化疗耐受组（0.401比0.556、0.085比0.205、0.316比0.499,均P＜0.05）.结论 CTS-B、CTS-L、uPA在NHL中高表达,与NHL的生物学行为密切相关;NHL患者CTS-B、CTS-L及uPA的联合检测及动态观察可作为NHL临床辅助诊断及预后判断的指标之一.

Objective To investigate mRNA expression levels of cathepsin B,cathepsin L and uPA in non-Hodgkin lymphoma （NHL） samples,and analyze the relationship between three genes and clinicopathological factors.Methods Real-time fluorescence quantitative RT-PCR was used to quantify the relative expression levels of cathepsin B,cathepsin L and uPA mRNA in 39 NHL patients without chemotherapy or radiotherapy and 15 patients with lymph node reactive hyperplasia.Relationship between their expression and clinico-pathological factors was analyzed.Results mRNA expression levels of cathepsin B,cathepsin L and uPA in NHL group were significantly higher than those in the control group （0.403 vs 0.128,0.209 vs 0.057,0.459 vs 0.031,all P 〈 0.05）.The mRNA expression levels of cathepsin B,cathepsin L and uPA were correlated with each other in NHL.The mRNA expression levels of cathepsin B,cathepsin L and uPA in advanced staged NHL were markedly higher than those in early staged NHL （0.453 vs 0.341,0.204 vs 0.085,0.372 vs 0.196,all P 〈 0.05）.mRNA levels of cathepsin B,cathepsin L and uPA were higher in NHL patients sensitive to chemotherapy than in those resistant to chemotherapy（0.401 vs 0.556,0.085 vs 0.205,0.316 vs 0.499,all P 〈 0.05）.Conclusions The high expression of cathepsin B,cathepsin L and uPA could be detected in NHL,and their expression has a close relationship with the biological behavior characters of NHL.The dynamic examination of cathepsin B,cathepsin L and uPA may be used as clinical auxiliary diagnosis and prognosis of NHL.