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神经型钙黏蛋白分子在多发性骨髓瘤患者中的表达 被引量:2

Expression of N-cadherin in patients with multiple myeloma
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摘要 目的 探讨神经型钙黏蛋白(N-cadherin)分子在多发性骨髓瘤(MM)患者中的表达及其临床意义.方法 收集54例初治MM患者及20名健康人骨髓单个核细胞,应用实时荧光定量PCR法检测N-cadherin mRNA表达,Western blot法测定其蛋白表达.结果 MM患者N-cadherin mRNA表达水平为6.056±3.033,健康对照组为1.788±0.778,二组相比差异有统计学意义(P<0.0001).按照ISS分期,Ⅰ期、Ⅱ期、Ⅲ期MM患者N-cadherin mRNA表达水平分别为3.681±3.185、5.757±3.292、7.460±3.647,其中Ⅱ期、Ⅲ期与健康对照组相比差异均具有统计学意义(均P<0.05);Ⅰ期与健康对照组相比差异无统计学意义(P>0.05).有髓外浸润及无髓外浸润患者N-cadherin mRNA表达量分别为9.159±3.178、5.083±3.288,差异有统计学意义(P< 0.001).有骨质破坏和无骨质破坏患者N-cadherin mRNA表达量分别为6.544±3.729、4.240±2.896,差异有统计学意义(P=0.047).初治MM患者N-cadherin mRNA表达水平为6.056±3.033,治疗后有效患者N-cadherin mRNA表达水平下降(3.768±2.216)(P=0.015),治疗无效患者N-cadherin mRNA水平与治疗前相比差异无统计学意义(P>0.05).初治MM患者N-cadherin蛋白表达量明显高于缓解期患者及健康对照组,而在治疗后复发的患者中,N-cadherin蛋白表达量明显高于初治患者.结论 N-cadherin分子在MM患者中高表达,其可能参与MM的发生及发展. Objective To investigate the expression and clinical significance of N-cadherin in patients with multiple myeloma (MM).Methods A total of newly diagnosed 54 MM patients and 20 controls were enrolled in this study.The expression of N-cadherin was detected by quantitative real-time polymerase chain reaction and Western blot.Results The N-cadherin mRNA expression levels of MM patients and normal controls were 6.056±3.033 and 1.788±0.778 (P 〈 0.000 1).N-cadherin mRNA expression levels of ISS stage Ⅰ,Ⅱ and Ⅲ were 3.681±3.185,5.757±3.292 and 7.460±3.647,respectively.The results indicated that N-cadherin was significantly up-regulated in stage Ⅱ and stage Ⅲ (P 〈 0.05),not in stage Ⅰ (P 〉 0.05) compared with controls.The median levels of N-cadherin mRNA expression in MM patients with or without extramedullary infiltration were 9.159±3.178 and 5.083±3.288,respectively (P 〈 0.001).MM patients with bone destruction had a higher N-cadherin mRNA expression level (6.544±3.729) than patients without bone destruction (4.240±2.896) (P =0.047).N-cadherin mRNA expression in newly diagnosed MM patients was 6.056±3.033,the expression in responded patients after treatment was significantly decreased (3.768±2.216) (P =0.015),and in no responded patients the levels of N-cadherin were not significantly decreased (P 〉 0.05).The levels of N-cadherin protein were up-regulated in newly diagnosed patients and relapsed patients compared with controls,which was reduced in remission MM patients.And relapsed patients had a higher N-cadherin protein expression than newly diagnosed patients.Conclusion High expression of N-cadherin in MM patients may play an important role during the occurrence and development of MM.
出处 《白血病.淋巴瘤》 CAS 2014年第8期456-459,464,共5页 Journal of Leukemia & Lymphoma
关键词 多发性骨髓瘤 神经型钙黏蛋白 Mutiple myeloma N-cadherin
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参考文献18

  • 1Pielarski KN,van Stegen B,Andreyeva A,et al.Asymmetric Ncadherin expression results in synapse dysfunction,synapse elimination,and axon retraction in cultured mouse neurons[J].PLoS One,2013,8:e54105.
  • 2Giampietro C,Taddei A,Corada M,et al.Overlapping and divergent signaling pathways of N-cadherin and VE-cadherin in endothelial cells[J].Blood,2012,119:2159-2170.
  • 3Maret D,Gruzglin E,Sadr MS,et al.Surface expression of precursor N-cadherin promotes tumor cell invasion[J].Neoplasia,2010,12:1066-1080.
  • 4Chung S,Yao J,Suyama K,et al.N-cadherin regulates mammary tumor cell migration through Akt3 suppression[J].Oncogene,2013,32:422-430.
  • 5Zhang B,Li M,McDonald T,et al.Microenvironmental protection of CML stem and progenitor cells from tyrosine kinase inhibitors through N-cadherin and Wnt-β-catenin signaling[J].Blood,2013,121:1824-1838.
  • 6Durie BG,Harousseau JL,Miguel JS,et al.International uniform response criteria for multiple myeloma[J].Leukemia,2006,20:1467-1473.
  • 7Nguyen PT,Kudo Y,Yoshida M,et al.N-cadherin expression is involved in malignant behavior of head and neck cancer in relation to epithelial-mesenchymal transition[J].Histol Histopathol,2011,26:147-156.
  • 8Zhi L,Wang M,Rao Q,et al.Enrichment of N-Cadherin and Tie2-bearing CD34+/CD38-/CD123+ leukemic stem cells by chemotherapyresistance[J].Cancer Lett,2010,296:65-73.
  • 9支蕾,王琳,田征,饶青,王敏,王建祥.N-cadherin在维持白血病干细胞特征中的作用[J].中国实验血液学杂志,2010,18(1):7-10. 被引量:6
  • 10何侃,于沛,邢海燕,李艳,田征,王敏,唐克晶,饶青.N-cadherin阳性白血病KG1a细胞系在G_0期抵抗VP16杀伤的作用[J].中国实验血液学杂志,2011,19(5):1102-1106. 被引量:4

二级参考文献20

  • 1Warner JK, Wang JC, Hope KJ, et al. Concepts of human leukemic development. Oncogene, 2004 ; 23 (43) : 7164 - 7 177.
  • 2Jordan CT, Guzman ML. Mechanisms controlling pathogenesis and survival of leukemic stem cells. Oncogene, 2004 ; 23 (43) : 7178 -7187.
  • 3Dean M, Fojo T, Bates S. Tumour stem cells and drug resisitance. Nat Rev Cancer, 2005 ; 5 (4) : 275 - 284.
  • 4Haug JS, He XC, Grindley JC, et al. N-cadherin expression level distinguishes reserved versus primed states of hematopoietic stem cells. Cell Stem Cell, 2008 ;2(4) :367 - 379.
  • 5Williams DA, Cancelas JA. Leukaemia: niche retreats for stem cells. Nature, 2006 ;444 (7121) :827 - 828.
  • 6Zhang J, Niu C, Ye L, et al. Identification of the haematopoiefic stem cell niche and control of the niche size. Nature, 2003 ; 425 (6960) :836 - 841.
  • 7Taichman RS. Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche. Blood, 2005 ; 105(7) :2631 -2639.
  • 8Wheelock MJ, Johnsony KR. Cadherin-mediated cellular signaling. Curr Opin Cell Biol, 2003, 15 (5) :509 -514.
  • 9Cavallaro U, Schaffhauser B, C hristofori G. Cadherins and the tumour progression: is it all in a switch? Cancer Lett, 2002; 176 (2) :123 -128.
  • 10Brabletz T, Jung A, Spaderna S, et al. Opinion : migrating cancer stem cells-an integrated concept of malignant tumour progression. Nat Rev Cancer, 2005 ; 5 (9) : 744 - 749.

共引文献8

同被引文献35

  • 1高峰,李艳,刘巍,卢香兰,李霞,王萍萍,刘云鹏.急性髓系白血病E-cadherin基因表达和CpG岛甲基化状态的研究[J].中华血液学杂志,2006,27(1):25-27. 被引量:8
  • 2Eide CA, 0丨Hare T. Chronic myeloid leukemia: advances inunderstanding disease biology and mechanisms of resistance totyrosine kinase inhibitors [ J ]. Curr Hematol Malig Rep, 2015,10(2): 158-166.
  • 3Radich JP,Dai H,Mao M,et al. Gene expression changes associatedwith progression and response in chronic myeloid leukemia [ J ]. ProcNatl Acad Sci US A, 2006’ 103(8): 2794-2799.
  • 4Fulga V, Rudico L,Balica AR, et al. Differential expression of e-cadherin in primary breast cancer and corresponding lymph nodemetastases [ J ]. Anticancer Res, 2015, 35 (2 ) : 759-765.
  • 5Park KS, Dubon MJ, Gumbiner BM. N-cadherin mediates themigration of MCF-10A cells undergoing bone morphogenetic protein4-mediated epithelial mesenchymal transition [ J ]. Tumour Biol,2015,36(5): 3549-3556.
  • 6Li N, Deng W, Ma J, et al. Prognostic evaluation of Nanog, 0ct4,Sox2,PCNA, Ki67 and E-cadherin expression in gastric cancer [ J ].Med Oncol,2015,32(1): 433.
  • 7He X,Li B, Shao Y, et al. Cell fusion between gastric epithelial cellsand mesenchymal stem cells results in epithelial-to-mesenchymaltransition and malignant transformation [ J ]. BMC Cancer, 2015,15: 24.
  • 8Kim YJ, Kang HB, Yim HS, et al. NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancerpatients [ J ] . Oncol Rep,2013,30 ( 4 ) : 1890-1898.
  • 9Alshenawy HA,Ali MA. Differential caveolin-1 expression in coloncarcinoma and its relation to E-cadherin-beta-catenin complex [ J ].Ann Diagn Pathol, 2013, 17 (6 ) : 476-482.
  • 10Colomiere M,Ward AC,Riley C, et al. Cross talk of signals betweenEGFR and IL-6R through JAK2 / STAT3 mediate epithelial-mesenchymal transition in ovarian carcinomas [ J ] . Br J Cancer,2009, 100(1 ); 134-144.

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