摘要
目的 探讨神经型钙黏蛋白(N-cadherin)分子在多发性骨髓瘤(MM)患者中的表达及其临床意义.方法 收集54例初治MM患者及20名健康人骨髓单个核细胞,应用实时荧光定量PCR法检测N-cadherin mRNA表达,Western blot法测定其蛋白表达.结果 MM患者N-cadherin mRNA表达水平为6.056±3.033,健康对照组为1.788±0.778,二组相比差异有统计学意义(P<0.0001).按照ISS分期,Ⅰ期、Ⅱ期、Ⅲ期MM患者N-cadherin mRNA表达水平分别为3.681±3.185、5.757±3.292、7.460±3.647,其中Ⅱ期、Ⅲ期与健康对照组相比差异均具有统计学意义(均P<0.05);Ⅰ期与健康对照组相比差异无统计学意义(P>0.05).有髓外浸润及无髓外浸润患者N-cadherin mRNA表达量分别为9.159±3.178、5.083±3.288,差异有统计学意义(P< 0.001).有骨质破坏和无骨质破坏患者N-cadherin mRNA表达量分别为6.544±3.729、4.240±2.896,差异有统计学意义(P=0.047).初治MM患者N-cadherin mRNA表达水平为6.056±3.033,治疗后有效患者N-cadherin mRNA表达水平下降(3.768±2.216)(P=0.015),治疗无效患者N-cadherin mRNA水平与治疗前相比差异无统计学意义(P>0.05).初治MM患者N-cadherin蛋白表达量明显高于缓解期患者及健康对照组,而在治疗后复发的患者中,N-cadherin蛋白表达量明显高于初治患者.结论 N-cadherin分子在MM患者中高表达,其可能参与MM的发生及发展.
Objective To investigate the expression and clinical significance of N-cadherin in patients with multiple myeloma (MM).Methods A total of newly diagnosed 54 MM patients and 20 controls were enrolled in this study.The expression of N-cadherin was detected by quantitative real-time polymerase chain reaction and Western blot.Results The N-cadherin mRNA expression levels of MM patients and normal controls were 6.056±3.033 and 1.788±0.778 (P 〈 0.000 1).N-cadherin mRNA expression levels of ISS stage Ⅰ,Ⅱ and Ⅲ were 3.681±3.185,5.757±3.292 and 7.460±3.647,respectively.The results indicated that N-cadherin was significantly up-regulated in stage Ⅱ and stage Ⅲ (P 〈 0.05),not in stage Ⅰ (P 〉 0.05) compared with controls.The median levels of N-cadherin mRNA expression in MM patients with or without extramedullary infiltration were 9.159±3.178 and 5.083±3.288,respectively (P 〈 0.001).MM patients with bone destruction had a higher N-cadherin mRNA expression level (6.544±3.729) than patients without bone destruction (4.240±2.896) (P =0.047).N-cadherin mRNA expression in newly diagnosed MM patients was 6.056±3.033,the expression in responded patients after treatment was significantly decreased (3.768±2.216) (P =0.015),and in no responded patients the levels of N-cadherin were not significantly decreased (P 〉 0.05).The levels of N-cadherin protein were up-regulated in newly diagnosed patients and relapsed patients compared with controls,which was reduced in remission MM patients.And relapsed patients had a higher N-cadherin protein expression than newly diagnosed patients.Conclusion High expression of N-cadherin in MM patients may play an important role during the occurrence and development of MM.
出处
《白血病.淋巴瘤》
CAS
2014年第8期456-459,464,共5页
Journal of Leukemia & Lymphoma