229例流产绒毛染色体核型分析及两种长期培养方法比较

Cytogenetic Study on the Chorionic Villi in 229 Cases of Early Spontaneous Abortus and Comparation of two Long-term Culture Methods

目的:探讨早期自然流产绒毛染色体核型分析在自然流产病因检测中的应用价值,并比较两种长期培养方法的差别。方法:选择孕早期自然流产的孕妇229例,在无菌条件下,从宫腔内取出绒毛,同时或单独经胰酶消化法与切碎贴壁法进行细胞培养,传代之后常规进行G显带,在显微镜下做核型分析。结果:229例流产胎儿绒毛,培养成功206例,成功率为89.96%。异常核型105例,异常率为50.97%,数目异常者101例,占异常核型的96.19%,以16三体最为多见。胰酶消化法的培养成功率及收获时间都显著优于直接贴壁法,差异具有统计学意义(P<0.05)。结论:自然流产绒毛染色体核型分析对流产查因具有实用价值。胰酶消化法较切碎贴壁法对流产绒毛长期培养及染色体核型分析更具实用性。

Objective： To investigate the application of karyotypes of chorionic villi in the first trimester abortus and compare trypsin digestion method with minced adherence method in the isolation and culture of chorionic villi samples in spontaneous abortions. Methods： A total of 229 cases of abortus were collected in sterile condition. Trypsin digestion method and minced adherence method were used respectively in chorionic villi cells isolation and the culture of these abortus. These chorionic villi cells were subcultrued. Their karyotypes were analysed by G band. Results： There were 105 cases with chromosomal abnormalities in the 206 successful cultured abortus over all 299 cases, There were 101 cases with chromosomal numberical abnormality, accounting for 96.19% of chromosomal abnormality. Trisomy 16 was the most common abnormality. Trypsin digestion method had higher success rate and shorter harvest time than minced adherence method （P〈0.05）. Conclusion： Karyotype analysis ofabortus villi was benefit to identify the abortion reason and could guide the next pregnancy. Trypsin digestion method used in cytogenetic study of chorionic villi samples in spontaneous abortions was more practicable and efficient.