摘要
目的:建立LC-MS/MS的分析方法测定大鼠血浆中的野黄芩苷,研究灯盏生脉胶囊中野黄芩苷在大鼠体内的药动学行为。方法:以噻氯匹定为内标,血浆样品经1%甲酸乙腈沉淀蛋白处理后,用LC-MS/MS法测定血浆中的野黄芩苷浓度。结果:野黄芩苷线性范围为1.31~670.00 ng·mL-1(γ>0.999),最低定量浓度为1.31 ng·mL-1,回收率、日内、日间考察均符合生物样品分析要求。实验结果显示,野黄芩苷在大鼠体内出现多峰现象。结论:建立的LC-MS/MS定量分析方法灵敏、准确,可用于大鼠血浆中野黄芩苷的测定及其药代动力学研究。
Objective: To develop a new LC-MS/MS method for determination of scutellarin of Dengzhanshengmai capsule in rat plasma. That method was applied to the pharmacokinetic study of scutellarin in rat. Methods: Ticlopidine was used as internal standard, plasma samples were determined by LC-MS/MS with acetonitrile protein precipitation. Results: The calibration curves were linear in the concentration range of 1.31 -670.00 ng. mL^-1 and the correlation coefficient was better than 0.999. The lower limit of quantitation was 1.31 ng. mL~. The recovery rate, within-day and between-day variances met the requirement of biological sample analysis. The experiment results showed that the drug concentration-time curve presented multi-peak phenomenon in rats. Conclusion: A sensitive and accurate method was successfully applied to the pharmacokinetic study of scutellarin in rat plasma.
出处
《现代生物医学进展》
CAS
2014年第28期5414-5418,5450,共6页
Progress in Modern Biomedicine
基金
广东省科技计划项目(2012B031800310)