摘要
目的:建立LC-MS/MS的分析方法测定大鼠血浆中的野黄芩苷,研究灯盏生脉胶囊中野黄芩苷在大鼠体内的药动学行为。方法:以噻氯匹定为内标,血浆样品经1%甲酸乙腈沉淀蛋白处理后,用LC-MS/MS法测定血浆中的野黄芩苷浓度。结果:野黄芩苷线性范围为1.31～670.00 ng·mL-1(γ>0.999),最低定量浓度为1.31 ng·mL-1,回收率、日内、日间考察均符合生物样品分析要求。实验结果显示,野黄芩苷在大鼠体内出现多峰现象。结论:建立的LC-MS/MS定量分析方法灵敏、准确,可用于大鼠血浆中野黄芩苷的测定及其药代动力学研究。
Objective： To develop a new LC-MS/MS method for determination of scutellarin of Dengzhanshengmai capsule in rat plasma. That method was applied to the pharmacokinetic study of scutellarin in rat. Methods： Ticlopidine was used as internal standard, plasma samples were determined by LC-MS/MS with acetonitrile protein precipitation. Results： The calibration curves were linear in the concentration range of 1.31 -670.00 ng. mL^-1 and the correlation coefficient was better than 0.999. The lower limit of quantitation was 1.31 ng. mL~. The recovery rate, within-day and between-day variances met the requirement of biological sample analysis. The experiment results showed that the drug concentration-time curve presented multi-peak phenomenon in rats. Conclusion： A sensitive and accurate method was successfully applied to the pharmacokinetic study of scutellarin in rat plasma.
出处
《现代生物医学进展》
CAS
2014年第28期5414-5418,5450,共6页
Progress in Modern Biomedicine
基金
广东省科技计划项目(2012B031800310)
