丹皮酚对TNF-α诱导的人牙龈成纤维细胞黏附分子表达水平的影响

Influence of Paeonol on the Expression of Adhesion Molecules on Human Gingival Fibroblasts

目的:研究丹皮酚(Paeonol,Pae)对TNF-α诱导的人牙龈成纤维细胞(human gingival fibroblasts,HGF)黏附分子表达水平的影响。方法:使用50 ng/mL的肿瘤坏死因子(tumor necrosis factor,TNF)-α诱导刺激人牙龈成纤维细胞,后分别加入浓度为0、50、100、200 mmol/L的Pae孵育培养2、4、6天后,用Western blot法检测细胞间黏附分子(intercellular adhesion molecule,ICAM)-1以及血管细胞黏附分子(vascularcell adhesion molecule,VCAM)-1的蛋白表达水平,用逆转录多聚酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测黏附分子的mRNA表达水平。结果:TNF-α诱导的人牙龈成纤维细胞ICAM-1和VCAM-1的蛋白和mRNA表达水平均显著高于空白对照组(P<0.05);不同浓度的丹皮酚可显著降低TNF-α诱导的人牙龈成纤维细胞ICAM-1和VCAM-1的蛋白和mRNA表达(P<0.05),且随Pae浓度的升高逐渐减少。当Pae浓度达到100 mmol/L,蛋白和mRNA表达水平的抑制效果达到稳定程度(P>0.05),当Pae浓度达到200 mmol/L时,ICAM-1和VCAM-1 mRNA抑制效果达到诱导前的水平(P>0.05)。诱导时间的长短对蛋白和mRNA表达水平抑制效果没有显著影响(P>0.05)。结论:丹皮酚可显著降低TNF-α诱导的人牙龈成纤维细胞黏附分子表达水平。

Objective： To investigate the influence of Paeonol on human gingival fibroblasts （HGF） stimulated by adhesion molecules. Methods： HGF were stimulated by 50 ng/mL tumor necrosis factor （TNF）-α, then different concentrations （0, 50, 100, 200 mmol/L） of Pae were added to the experimental group. The protein and mRNA expression of intercellular adhesion molecule （ICAM）-1 and vascular cell adhesion molecule （VCAM）-1 were examined by western blot and reverse transcription polymerase chain reaction （RT-PCR）. Results： The protein and mRNA expression of ICAM-1 and VCAM-1 were increased by TNF-α stimulation （P〈0.05）, while Pae inhibited the expression oflCAM-1 and VCAM-1 with the increase of Pae concentration （P〈0.05）. There was no significant effect of inhibition on the expression of the protein and mRNA levels in induction time （P〉0.05）. Conclusion： Paeonol had the potential to provide new method for the treatment of periodontal disease.