电休克对大鼠空间记忆及海马p-ERK活性的影响

The Effects of ECT on Spatial Memory and p-ERK of Rats

目的:观察电休克对大鼠空间记忆和海马磷酸化细胞外调节蛋白激酶(p-ERK)活性的影响。方法:大鼠随机分为电休克组和伪电休克组,每组12只。电休克组每天给予电痉挛刺激,伪电休克组每天给予假电痉挛刺激,共10天;第11天用水迷宫检测各组大鼠的空间学习记忆,然后每组大鼠再随机分为两组,每组6只。一组于学习后1小时处死取海马用Western blot法检测p-ERK活性,另一组于48小时后行水迷宫空间位置探寻实验检测大鼠的存储记忆。结果:电休克组的潜伏期显著长于伪电休克组(P<0.01)。电休克组在隐匿平台周围区域/相反区域的搜寻时间无显著性差异(P>0.05);伪电休克组在隐匿平台周围区域/相反区域的搜寻时间有显著性差异(P<0.05)。电休克组海马p-ERK活性较伪电休克组显著下降(P<0.01)。结论:电休克可导致大鼠显著空间记忆障碍,海马p-ERK活性的降低可能是其机制之一。

Objective： To investigate the effects of Electroshock therapy （ECT） on spatial memory and phosphorylated extracellular-signal related kinase （p-ERK） of rats. Methods： Rats were randomized into ECT group and sham ECT group. Each group consisted of 12 rats. ECT group received true ECT and sham group received sham ECT. The true ECT or sham ECT was administered once each day, ten days in a whole. At the llth day the rats were trained and tested in Morris water maze localization navigation experiment. Then each group was randomized into two groups again and each group had 6 rats. One group was decapitated 1 hour after training. Their hippocampus was taken quickly for detecting p-ERK expression by western blot. The other group received probe tests of Morris water maze 48 hours after training. Results： The latency of ECT group was significantly longer than that of sham ECT group （P〈 0.01）. There was no significant difference in the search time around the hidden platform and the time in the opposite area in ECT group （P〉 0.05）. But there was significant difference between the search time around the hidden platform and the time in the opposite area in sham ECT group （P〈0.05）. The p-ERK1/2 level of ECT group was significantly lower than that of sham ECT group （P〈 0.01）. Conclusions： ECT can result in significant amnesia for spatial memory. P-ERK may play an important role in the development of spatial memory impairment caused by ECT.