RP-HPLC法测定哈蟆油中胆固醇棕榈酸酯含量

Determination of cholesterol palmitate in oviductus ranae by RP-HPLC

目的运用RP-HPLC法测定哈蟆油中胆固醇棕榈酸酯的含量。方法使用Agilent zorbax-C18色谱柱（4.6 mm×150 mm,5μm）;流动相∶甲醇-水（79∶21）;柱温：30℃;体积流速：1.5 mL/min;检测波长：210nm;进样量均为10μL。结果哈蟆油中胆固醇棕榈酸酯进样量在0.188 4~0.502 4μg范围内呈良好的线性关系,平均回收率为100.61%,RSD值为1.01%,且重复性、精密度、稳定性均良好。结论此方法可以通过RP-HPLC法快速、稳定、精密的测定哈蟆油中胆固醇棕榈酸酯的含量,减少以往利用正相色谱的不便。

Objective To determine the contents of Cholesterol palmitate in Oviductus Ranae by RP-HPLC.Methods The chromatographic column： Agilent zorbax-C18 （4.6 × 150 mm, 5 μn） ; The mobile phase： methanol-water（79 ： 21 ） ; The column temperature ： 30 ℃ ; The volumetric flow rate： 1.5 mL/min; The UV detector was set at 210 nm; The sample size： 10μL. Resuits Cholesterol palmitate showed good liner relation when the content was during 0. 188 4 - 0. 502 4μg in the samples, the average recovery was 100.61%, RSD = 1.01%, good stability, precision and repeatability; Conclusion This method can determine the contents of Cholesterol palmitate in Ranae Oviductus, it is fast, accurate and stable and reduce the use of normal phase chromatography inconvenience in the past.